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Scientist I, Research
4 months ago
Overview
The Scientist I is responsible for developing next-generation tumor-infiltrating lymphocyte (TIL) approaches for therapeutic applications. Such methods include the use of knockout approaches, ex vivo manipulation of TILs (tumor-infiltrating lymphocytes) during the expansion process, and other techniques aimed at improving the metabolic function, trafficking, and persistence of TILs. To be successful in this role, you will need to have experience working in a research laboratory, using high-throughput screening techniques to assess the metabolic function of T cells with CRISPR/Cas9 technology, next-generation sequencing, and single-cell sequencing technologies. This role requires individuals to be very motivated and able to bring impactful work to the team and help move new TIL approaches into the clinic.
Essential Functions and Responsibilities
- Designs, plans, and leads experimental coordination, including scheduling, preparing/sourcing materials/media/buffers, and other consumables/reagents.
- General molecular biology experiments such as ligations, restriction enzyme digests, PCR, etc.
- Generate TIL and maintain primary and tumor cell lines.
- Generate transient and stable primary cells and tumor cell lines using various techniques, including electroporation and viral transduction.
- Optimize gene integration and gene editing techniques.
- Assess the functionality of metabolically reprogrammed TIL and evaluate their therapeutic potential in vitro and in vivo. These include high throughput assessment of mitochondrial function and metabolic state and performing functional assays such as cytokine release and cytotoxicity assays.
- Support Research, take on independent projects, and support team projects.
- Communicates findings effectively through presentations, reports, and scientific publications.
- Must adhere to Iovance Biotherapeutics’ core values, policies, procedures, and business ethics.
- Comply with Safety SOPs (standard operating procedures), SDS sheets, and laboratory procedures per company policy and OSHA (Occupational Safety and Health Administration) regulations.
Required Education, Skills, and Knowledge
- Requires Ph.D. in Immunology, Cell Biology, or a related field with 2+ years of relevant experience. Or Master in Immunology, Cell Biology, or a related field with 8-12 years of relevant experience.
- Deep experience in mitochondrial biology and metabolism
- Familiarity with CRISPR/Cas9 technology, high-throughput screening techniques, next-generation sequencing, and single-cell sequencing technologies.
- Prior experience with molecular biology and mammalian cell culture techniques.
- Success working in cross-disciplinary, highly communicative teams.
- Experience with flow cytometry and basic knowledge of T-cell immunology.
The physical demands described here represent those that an employee must meet to perform the essential functions of this job successfully. Reasonable accommodation may be made upon request to enable individuals to perform essential functions. Please contact Human Resources to request accommodation.
Physical Demands and Activities Required
- Must be able to move to and from the office to the lab located on separate floors and move about the office and lab as needed.
- Must be able to remain in a stationary position, standing or sitting for prolonged periods of time.
- Must be able to grasp with both hands, perform repetitious actions such as pinch with thumb and forefinger, turn with hand/ arm, and reach above shoulder height.
- Must be able to use several types of laboratory equipment and pipettes for extended periods.
- Must be able to work on a tablet or computer typing for 50% of a typical working day.
- Must be able to move and lift 20 pounds multiple times daily.
- Must be able to use near vision to view samples at close range.
- Wear proper personal protective equipment, such as safety glasses, lab coats, and closed-toed shoes, when in a laboratory setting.
Mental: Clear and conceptual thinking ability; excellent judgment, troubleshooting, problem-solving, analysis, and discretion; ability to handle work-related stress; ability to handle multiple priorities simultaneously; and ability to meet deadlines
Work Environment
This position will work in both an office and a laboratory setting. When in the lab, there may be a risk of exposure to hazardous or biological waste within the environment through receipt, transport, storage, preparation, dispensing, administration, cleaning, and/or disposal of contaminated areas and waste.
The statements contained in this document are intended to describe the general nature and level of work being performed by a colleague assigned to this description. They are not intended to constitute a comprehensive list of functions, duties, or local variances. Management retains the discretion to add or change the position's responsibilities at any time.
Iovance is committed to cultivating and offering a diverse and inclusive work environment. As an equal-opportunity employer, our employees and applicants will be considered without regard to an individual’s race, color, religion, sex, pregnancy, national origin, age, physical and mental disability, marital status, sexual orientation, gender identity, gender expression, genetic information, military and veteran status, and any other characteristic protected by applicable law. If you need assistance or accommodation to apply for one of our opportunities, please contact careers@iovance.com.
By voluntarily providing information and clicking “Submit Application”, you explicitly consent to the collection and use of your personal information for the purposes described above and in our Candidate Privacy Notice.
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