Research Associate/Senior Research Associate, Immunohistochemistry

1 month ago

South San Francisco, California, United States SonoThera Full time

Location: South San Francisco, CA

Status: Regular Full-Time

Reports To: Associate Director, Gene Therapy

About SonoThera:

SonoThera is a venture capital-backed early-stage biopharmaceutical company developing ultra-sound-guided non-viral targeted gene therapies to treat multiple diseases of high unmet need. The Gene Therapy group at SonoThera uses a collaborative, interdisciplinary, and forward-thinking approach to solving many of the current challenges facing genetic medicine discovery and drug development. SonoThera is headquartered in South San Francisco, CA.

Job Description:

The Gene Therapy group at SonoThera is seeking to hire a Research Associate/Senior Research Associated to become an integral part of the gene therapy drug development effort. We seek candidates who think critically and have the desire and ability to learn new techniques. This position requires adaptability and an ability to perform at a high level in a fast-paced environment. A qualified candidate will have broad technical experience in immunohistochemistry and image analysis. In this position, you will play an integral role in characterizing the expression of transgenic constructs animal tissues and in vitro cellular models. You will develop and optimize protocols for antibody staining for detection of transgene and cell marker expression, as well as develop and perform quantitative image analysis methods.

Qualifications:

Bachelor's or Master's degree in the biological sciences (e.g. Genetics, Biochemistry, Molecular and Cellular Biology, Physiology, or related field) with 2-5 years of relevant postgraduate research experience in an industry setting.

Creativity and the ability to work within an interdisciplinary team to achieve technical and corporate milestones.

Strong verbal and written communication, critical thinking, and problem-solving skills.

Technical Requirements and Skills:
Proven track record of developing and performing chromogenic and fluorescence immunohistochemistry staining, imaging, and analysis for multiple targets.

Extensive experience with antibody screening and selection for reporter expression and cell-type identification.

Experience working with multiple tissue preparations (FFPE, fixed-frozen, fresh-frozen, etc.) and multiple tissue types (mouse and NHP).

Previous experience using high-throughput methods including autostainers (i.e. Leica Bond) and slide scanners (i.e. Evident VS200).

Expertise with quantitative image analysis software including Visiopharm, HALO, Imaris, ImageJ, QuPath. Familiarity with image analysis automation is a plus.

Experience in generating data summary plots (i.e. GraphPad Prism), as well as slide preparation and presentation.

Ideal candidate will have familiarity with ELNs (i.e. Benchling), RNAscope/ISH, ddPCR, qPCR, Olink-based proteomics, MSD, protein isolation, Western blot.

Strong record-keeping and organizational skills.

Compensation: The salary range for this temporary position is $95,000.00 to $120,000.00 USD hour. This salary range is an estimate, and the actual salary may vary based on various factors, including, without limitation, individual education, experience, tenure, skills, and abilities, as well as internal equity and alignment with market data, including potential adjustments for geographic location.

Equal Opportunity for Diversity & Inclusion

SonoThera provides equal employment opportunities to all employees and applicants for employment and prohibits discrimination and harassment of any type without regard to race, color, religion, age, sex, national origin, disability status, genetics, protected veteran status, sexual orientation, gender identity or expression, or any other characteristic protected by federal, state or local laws.

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