Protein Purification Senior Scientist

2 weeks ago

Reston, Virginia, United States Pledge Therapeutics Full time

Job Title: Senior Scientist/Protein Purification

Location: Canton, MA

Job Summary:

We are seeking an experienced Senior Scientist to lead protein purification, production, and characterization efforts within our Rapid Response Group (RRG). The RRG operates with a hands-on, mission-focused approach, driving research on protein targets and therapeutic development. The successful candidate will be a key member of this elite team, driving research and development of critical tools and methodologies.

Key Responsibilities:

  • Develop and optimize protein expression systems, focusing on baculovirus (insect cell) and/or mammalian systems.
  • Design and implement novel purification protocols to address technical challenges, leveraging a deep understanding of molecular biology and biochemistry.
  • Troubleshoot and refine protein expression and purification strategies, ensuring high-quality protein production through advanced chromatographic and filtration techniques.
  • Analyze experimental data, support scale-up processes, and drive continuous improvement while ensuring adherence to timelines in a dynamic, fast-paced environment.
  • Collaborate cross-functionally with immuno-oncology, virology, and structural biology teams, sharing expertise to enhance our ability to work with a diverse family of proteins.

Qualifications:

  • PhD in Biochemistry, Cell Biology, or a related field; exceptional MS candidates will be considered.
  • 5-7 years of relevant experience in a dynamic, fast-paced environment.
  • Expertise in construct design, sub-cloning, and protein purification, with experience spanning the entire protein production pipeline, from gene design through to characterization.
  • Deep expertise and hands-on experience with multiple protein expression systems, particularly baculovirus and/or mammalian expression, evidenced by a track record of prior work across different protein classes.
  • Experience in purifying biologics using techniques such as affinity chromatography (e.g., Protein A/G/L, Ni-NTA), ion exchange, size exclusion, and hydrophobic interaction chromatography.
  • Proficiency in analytical techniques such as SDS-PAGE, HPLC or UHPLC, and other relevant analytical methods.
  • Excellent troubleshooting skills, strong analytical abilities, meticulous attention to detail, and exceptional organizational skills.
  • High degree of independence, intellectual capacity, and initiative.
  • Strong work ethic and adaptability, with the ability to multitask and maintain a can-do attitude as R&D priorities shift.

Job Type: Full-time, Work Permit, Equal Opportunity Employment.

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