Scientist II-Antibody Technologies

4 weeks ago

San Diego, United States Indotronix International Corporation Full time

Job Title: Scientist II-Antibody Technologies

Location: San Diego, CA 92121

Duration: 12 months

Shift Hours: 8 AM to 5 PM PST


Job Description:

  • 3 Must haves on the resume: Min. 2 years lab experience, Flow cytometry experience, and Tissue culture experience.

We are seeking a highly motivated and organized Scientist II to contribute to the research and development of monoclonal antibodies in our Antibody Technologies team. This individual will be responsible for working in a dynamic team environment and be a contributor to multiple work streams, including but not limited to testing new antibodies for use in flow cytometry applications, hybridoma tissue culture and development, recombinant antibody expression. The position requires extensive hands-on experience with flow cytometry, expertise in tissue culture, and an understanding of biochemistry and molecular biology. The successful candidate is expected to set up and execute experiments, be detail oriented, skilled at data analysis and troubleshooting and will be accountable for preparing and presenting data at lab meetings with guidance regarding required tasks and expected results.

Duties and responsibilities:

  • Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry.
  • Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.
  • Responsible for transfection and expression of antibodies and recombinant proteins in cell lines, hybridoma tissue culture, and culture of various cell lines for testing. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques.
  • Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.
  • Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload.
  • Involvement with scheduling, operational and maintenance duties required within the laboratory.

Knowledge and skills:

  • Hands on experience designing, performing, and analyzing flow cytometry experiments, preferably on flow cytometers (LSRII, LSR Fortessa, Canto, Aria).
  • Proficient in tissue culture techniques; including transfection of cells for protein expression.
  • Software: FACS Diva, FlowJo, FCS Express, and Microsoft Office Suite.
  • Familiarity with molecular biology (DNA preparation)
  • Preferred: Familiarity with assay automation and automated liquid handlers.
  • Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.
  • Effective written and oral communication skills.
  • Demonstrated ability to follow established policies and procedures.
  • Demonstrated ability to keep neat, accurate, and complete records.
  • Effective analytical and problem-solving skills.

Education and experience:

  • A Bachelor’s or Master of Science degree in cellular or molecular biology, immunology, cell biology or related scientific discipline and a minimum of 2-5 years of laboratory experience required.
  • Flow cytometry and/or tissue culture experience required.

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