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Scientist

1 month ago

Bothell, United States Integrated Resources, Inc ( IRI ) Full time

Title: Research Associate III, Antibody Discovery & Engineering


Location: Bothell, WA


Duration: 8 months


Job Description:

  • Supports a broad variety of research studies to assess the value of health care interventions.
  • Works with project teams to manage the day-to-day activities of studies.
  • Collaborates with Medical and Marketing staff to ensure consistent Outcomes Research messages.
  • Database analysis, chart reviews, patient surveys, literature reviews, decision models, budget impact models, systematic reviews, meta-analyses, product strategic planning, and cost-effectiveness analyses.
  • Assists in the process of abstracts/posters, PowerPoint presentations and publications.
  • Manages relationships/coordinates activities such as advisory panels and best practice sharing with users of the program.
  • Participate in development of antibody-based oncology therapeutics.
  • The Scientist will report to the Molecular and Cellular Technologies team lead within Antibody Discovery.
  • Their primary responsibilities will include engineering cell lines and performing flow-based assays to support antibody campaigns.
  • The successful candidate will work well in a highly collaborative team environment and possess expertise in flow cytometry, mammalian cell culture, and cell engineering.



Responsibilities:

  • Design, execute, troubleshoot, optimize, and interpret experiments.
  • Order constructs for overexpression and knockout cell line generation.
  • Engineer cell lines using transfection, nucleofection, CRISPR/Cas9, lentiviral transduction, and other methodologies.
  • Evaluate target expression on engineered and endogenous cell lines using flow cytometry and molecular characterization.
  • Perform routine cell culture maintenance and banking, working with both primary and cancer cells.
  • Optimize and perform flow cytometry assays to screen and characterize antibodies.
  • Maintain laboratory spaces, equipment, and supplies.
  • Keep an up-to-date and accurate electronic laboratory notebook.
  • Present experimental findings and contribute to scientific discussion at group and cross-functional team meetings.



Required Qualifications:

  • Bachelor’s degree + 4 years laboratory research experience or master's degree + 2 years laboratory research experience in biological sciences or related subject.
  • Proficiency in culturing/maintaining multiple cell lines under BSL2 conditions.
  • Knowledge of cell engineering platforms, including transient transfection, generation of stable cell lines, and CRISPR.
  • Experience in multi-color flow cytometry.



Preferred Qualifications:

  • Experience with FACS and single cell cloning.
  • Basic molecular biology skills, including vector design, cloning, and PCR.
  • Knowledge of antibodies or antibody drug conjugates (ADCs).