NGS-Based Molecular Diagnostic Assay Specialist

21 hours ago


Boston, Massachusetts, United States Foundation Medicine Full time

About the Role

The Senior Research Associate, Assay Development supports the development, design, and validation of Next Generation Sequencing (NGS)-based molecular diagnostic assays for internal and external research projects. As a Senior Research Associate position, the incumbent is responsible for leveraging additional years of experience and/or education for a more advanced level of analysis and research activities.

Key Responsibilities

  • Prepare and isolate nucleic acid from oncology specimens (such as DNA/RNA extraction from FFPE tissues and blood samples).
  • Perform standard molecular biology techniques (such as enzymatic manipulation of DNA and RNA).
  • Perform specialized and complex experiments in such areas of Next Generation Sequencing.
  • Operate complex laboratory equipment such as high throughput liquid handlers and Illumina sequencers.
  • Support the timely processing and reporting of oncology samples to internal and external collaborators.
  • Manage experimental data in a computer database (e.g. LIMS).
  • Analyze and troubleshoot research test results.
  • Maintain detailed notes of experimental data with proper documentation.
  • Prepare reports and presentations to communicate results in group meetings.
  • Develop and follow SOPs.
  • Train and mentor other team members on standard operating procedures.
  • Participate in training sessions and workshops.
  • Independently perform lab testing.

Requirements

  • Bachelor's Degree in Molecular Biology or a related life science field and 1+ year of life science research experience in industry, a clinical research organization, or a hospital.
  • Master's Degree in Molecular Biology or a related life science field.
  • Experience working with typical oncology specimens including Formalin-fixed, Paraffin-embedded (FFPE) tissue samples, blood, bone marrow.
  • Experience working with standard molecular biology techniques (nucleic acid isolation and analysis, Polymerase Chain Reaction (PCR), qPCR, and enzymatic manipulation of DNA and RNA).
  • Experience with high throughput automation and liquid handling equipment's (such as Hamilton Star, KingFisher).
  • Demonstrate good understanding of the practices and principals of NGS, including DNA-seq and RNA-seq protocols, and various target-enrichment approaches e.g. multiplex-PCR, hybrid selection.
  • Demonstrated ability to effectively communicate, both written and verbal, scientific concepts, experimental plans, and results.
  • Proficiency in Microsoft Office suite, particularly Word, Power Point, Excel.
  • Proficiency in data visualization and statistical software (such as JMP).
  • Understanding of HIPAA and importance of privacy of patient data.
  • Commitment to reflect FMI's values: passion, patients, collaboration, and innovation.


  • Boston, Massachusetts, United States Foundation Medicine Full time

    About the RoleThe Senior Research Associate, Assay Development supports the development, design, and validation of Next Generation Sequencing (NGS)-based molecular diagnostic assays for internal and external research projects at Foundation Medicine. This role requires leveraging additional years of experience and/or education for a more advanced level of...


  • Boston, Massachusetts, United States Dana-Farber Cancer Institute Full time

    Position OverviewThe Molecular Genetics Specialist plays a vital role within the Dana-Farber Cancer Institute's Molecular Biology Core Facilities, focusing on the quality assessment of Next-Generation Sequencing (NGS) samples, preparation of libraries, and conducting sequencing evaluations.This position requires strict adherence to the regulations and...


  • Boston, Massachusetts, United States Dana-Farber Cancer Institute Full time

    Position OverviewThe Molecular Genetics Specialist is responsible for performing quality control, library preparation, and sequencing analysis within the Molecular Biology Core Facilities. This role involves the operation, calibration, and maintenance of Next-Generation Sequencing (NGS) instruments, ensuring compliance with established regulations and...

  • Genomics Specialist

    1 week ago


    Boston, Massachusetts, United States Dana-Farber Cancer Institute Full time

    Job SummaryThe Genomics Technologist plays a vital role in the Molecular Biology Core Facilities at Dana-Farber Cancer Institute, performing Next-Generation Sequencing (NGS) sample quality control, library preparation, and sequencing analysis. This position requires adherence to applicable regulations and standards established by DFCI and EH&S, as well as...


  • Boston, Massachusetts, United States Orchard Therapeutics Full time

    About the RoleOrchard Therapeutics is seeking a highly skilled and experienced professional to join our Clinical Development Team as a Director, Clinical Bioanalytical Diagnostic Development. This is an exciting opportunity to lead the development of bioanalytical strategies and assays for our gene therapy products.Key ResponsibilitiesDevelop and implement...


  • Boston, Massachusetts, United States Dana-Farber Cancer Institute Full time

    Job SummaryThe Genomics Technologist plays a vital role in the DFCI Molecular Biology Core Facilities, performing Next-Generation Sequencing (NGS) sample quality control, library preparation, and sequencing analysis. This position requires adherence to applicable regulations and standards established by DFCI and EH&S, as well as operation, calibration, and...


  • Boston, Massachusetts, United States Dana-Farber Cancer Institute Full time

    Position SummaryThe Molecular Biology Technologist is integral to the operations within the Molecular Biology Core Facilities, focusing on Next-Generation Sequencing (NGS) sample assessment, library preparation, and sequencing evaluation.In compliance with the relevant regulations and standards, the Molecular Biology Technologist is responsible for the...


  • Boston, Massachusetts, United States Manifold Bio Full time

    Manifold Bio is a biotech company pursuing a pipeline of protein therapeutics using novel molecular measurement technologies and library-guided protein engineering. Our drug discovery engine is differentiated by massively parallel screening in vivo from the beginning of our discovery process. This unique platform is powered by a proprietary protein barcoding...


  • Boston, Massachusetts, United States Kojin Therapeutics Full time

    We are seeking a dedicated and innovative scientist to enhance our preclinical pharmacology division.The ideal candidate will possess a robust foundation in molecular pharmacology pertinent to oncology or other medical conditions, demonstrated expertise in exploring disease mechanisms and drug interactions, as well as proficiency in employing various...


  • Boston, Massachusetts, United States Kojin Therapeutics Full time

    We are seeking a dynamic and curious scientist to enhance our preclinical pharmacology division.The ideal applicant will possess a robust foundation in molecular pharmacology, particularly in relation to oncology or similar conditions. Proven expertise in exploring disease mechanisms and drug interactions, along with proficiency in various molecular...


  • Boston, Massachusetts, United States Foundation Medicine, Inc. Full time

    Foundation Medicine, Inc. Full TimeAbout the JobThe Senior Manager, Regulatory Affairs is responsible for supporting the regulatory function and its activities, the development of regulatory strategy, and contributing to the regulatory approval process for FMI products. FMI's products include diagnostic assays that provide molecular insights to physicians to...


  • Boston, Massachusetts, United States Verve Therapeutics, Inc. Full time

    Lead Research Scientist - Preclinical PharmacologyVerve Therapeutics, Inc. is an innovative biotechnology firm focused on tackling worldwide cardiovascular health issues. With an emphasis on gene-editing advancements, Verve is committed to transforming the landscape of cardiovascular disease treatment. The organization’s pioneering initiatives aim at...


  • Boston, Massachusetts, United States Kojin Therapeutics Full time

    We are seeking a dedicated and innovative scientist to enhance our growing preclinical pharmacology division.The ideal candidate will possess a robust background in molecular pharmacology pertinent to oncology or related conditions, with demonstrated expertise in exploring disease mechanisms and drug interactions. Proficiency in employing diverse molecular...


  • Boston, Massachusetts, United States Kojin Therapeutics Full time

    We are seeking a dedicated and innovative scientist to enhance our growing preclinical pharmacology division.The ideal candidate will possess a robust foundation in molecular pharmacology pertinent to oncology or other medical conditions, with demonstrated expertise in exploring disease mechanisms and drug interactions. Proficiency in various molecular...


  • Boston, Massachusetts, United States Kojin Therapeutics Full time

    About Kojin TherapeuticsKojin Therapeutics, Inc. is at the forefront of innovative research in cell-state and ferroptosis biology, aiming to create pioneering therapies for various cancers and other serious health conditions. Our Boston-based company is named after the Japanese deity symbolizing the control of fire for humanity's benefit. We have established...


  • Boston, Massachusetts, United States Brigham and Women's Hospital Full time

    Position Overview The Division of Transplant Surgery and The Transplant Surgery Research Laboratory at Brigham and Women's Hospital is in search of a distinguished PhD-level research specialist with a strong background in molecular and cellular biology, particularly in the realms of immunology, aging, or similar disciplines. This role will focus on...


  • Boston, Massachusetts, United States Foundation Medicine Full time

    About the JobThe Regulatory Affairs Manager is responsible for supporting the regulatory function and its activities, the development of regulatory strategy, and contributing to the regulatory approval process (including US FDA and other regulatory authorities) for Foundation Medicine's products. Foundation Medicine's products include diagnostic assays that...


  • Boston, Massachusetts, United States Mass General Brigham Full time

    Job SummaryWe are seeking a highly skilled MLS Technical Specialist to join our team at Mass General Brigham. The successful candidate will be responsible for providing technical expertise and support to our laboratory operations.Key ResponsibilitiesProvide technical oversight and guidance to laboratory staff, ensuring compliance with regulatory requirements...


  • Boston, Massachusetts, United States Nona Biosciences Full time

    Position OverviewKey ResponsibilitiesDevelop and sustain mammalian cell lines for various research initiativesExecute mammalian cell transfections, produce lentiviruses, and perform transduction processesConduct flow cytometry for binding assays, immunophenotyping, and functional assays based on cellsUtilize ELISA/MSD techniques to measure cytokine...


  • Boston, Massachusetts, United States Nona Biosciences Full time

    Position OverviewKey ResponsibilitiesDevelop and sustain mammalian cell lines for various projects.Execute mammalian cell transfections, along with lentivirus production and transduction processes.Conduct flow cytometry for binding assays, immunophenotyping, and functional assays based on cells.Utilize ELISA/MSD techniques to measure cytokine concentrations...