Junior Research Associate
2 weeks ago
We are seeking a highly motivated and detail-oriented Junior Specialist/Assistant Specialist to join our dynamic research team in the Krogan lab at the University of California, San Francisco. The successful candidate will have the opportunity to work on cutting-edge projects in molecular biology, virology, and proteomics, focusing on mapping protein interactions and pathways involved in Human Immunodeficiency Virus (HIV) infection and latency.
Key Responsibilities- Conduct molecular biology techniques, including molecular cloning, PCR, and western blotting.
- Work with cell/tissue culture and perform affinity purifications of proteins.
- Develop and implement new protocols for gene-editing and mass spectrometry-based proteomics experiments.
- Prepare, sequence, and analyze next-generation sequencing libraries to support CRISPR screening experiments.
- Isolate and culture primary human immune cells, and perform functional analysis via Sanger sequencing and flow cytometry.
- Purify infectious HIV and infect T cells with HIV in a biosafety level-3 facility.
- Bachelor's degree (or equivalent degree) or at least four years of research experience for Junior Specialist.
- Master's degree or a bachelor's degree with three or more years of research experience for Assistant Specialist.
- Experience in essential molecular biology techniques, cell/tissue culture, and ability to work independently and meet deadlines.
- Effective verbal and written communication skills to interface with the team.
- Willingness to work with infectious HIV virus in a BSL-3 facility and ability to learn new methods and skills.
- Experience with transfection or viral transduction, affinity purifications of proteins, CRISPR/gene editing techniques, immunoprecipitation, and mass spectrometry data analysis.
- Experience with managing large datasets and/or databases, and statistical data analysis.
The University of California, San Francisco offers a competitive salary range of $51,300-$65,900, depending on qualifications and experience. We also offer a comprehensive benefits package, including medical, dental, and vision insurance, as well as a retirement plan.
Please apply online with a CV, cover letter, and three references. We are an equal opportunity employer and welcome applications from diverse candidates.
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