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Research Associate I – Stem Cells, Gene Editing and Differentiation

3 months ago

Seattle, United States Allen Institute for Cell Science Full time

The mission of the Allen Institute is to unlock the complexities of bioscience and advance our knowledge to improve human health. Using an open-science, multi-scale, team-oriented approach, the Allen Institute focuses on accelerating foundational research, developing standards and models, and cultivating new ideas to make a broad, transformational impact on science.

The mission of the Allen Institute for Cell Science is to create multi-scale visual models of cell organization, dynamics, and activities . Our approach encompasses large scale data collection, observation, theory, and predictions to understand cellular behavior in normal and pathological contexts.

The Stem Cells and Gene Editing group within the Experimental Cell Science team is seeking a proactive, collaborative, and curious Research Associate to join our multi-disciplinary group. This role involves gene editing as well as maintaining and characterizing CRISPR-Cas9 edited human induced pluripotent stem cell (hiPSC) lines on an as-needed basis. These cell lines are crucial for internal research and are also distributed worldwide as part of the Allen Cell Collection. Additionally, this Research Associate will employ various methods to differentiate hiPSC lines to several mesenchymal cell lineages. This work will advance the mission of the institute by supplying cells in various states to dynamic live-cell imaging experiments intended to interrogate those states.

The Allen Institute believes that team science significantly benefits from the participation of diverse voices, experiences, and backgrounds. High-quality science can only be produced when it includes different perspectives. We are committed to increasing diversity across every team and encourage people from all backgrounds to apply for this role.

Essential Functions

Maintain human stem cell cultures including feeding, passaging and freezing with strict adherence to internal SOPs, GLPs and regulatory requirements Assist in performing differentiation experiments Employ CRISPR/Cas9-based and related gene editing methods to create novel engineered cell lines for specific cell science projects Prepare laboratory reagents Routinely perform molecular biology experiments using PCR based technologies Precisely follow protocols and workflows for gene editing and cell culture Properly handle hazardous chemicals Maintain accurate and detailed laboratory notebook records Perform laboratory inventory functions Prepare written and oral reports on a regular basis Assist in cross-functional activities including hand-offs to various teams

Note: Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. This description reflects management’s assignment of essential functions; it does not proscribe or restrict the tasks that may be assigned.

Required Education and Experience

Bachelor’s Degree in cell biology, or a related biological life science

Preferred Education and Experience

0 – 2 years of relevant post-undergraduate experience Strong background in cell biology Experience with laboratory practices related to human stem cell culture or differentiation Strong interpersonal and organization skills, careful attention to detail, and ability to work both independently and collaboratively

Physical Demands

Occasional lifting to 30 pounds Fine motor movements in fingers/hands to operate computers and other office equipment; repetitive motion with lab equipment

Position Type/Expected Hours of Work

This role is currently working onsite and is expected to work onsite for the majority of working hours. The primary work location for this role is 615 Westlake Ave N., with the flexibility to work remotely on a limited basis.