Research Associate III, Molecular Biology
1 week ago
HDT Bio is pioneering technologies that direct the host immune system to treat or prevent cancer and infectious diseases. The research associate will be primarily responsible for working on the development and characterization of our RNA technology. You will have the opportunity to work closely with molecular biologists, virologists, immunologists, and material scientists to optimize formulation and delivery of RNA with specific applications in areas of vaccine and therapeutic development for infectious disease and oncology applications.
A background in molecular biology is required. Major techniques include cloning, RNA transcription/capping, analysis of DNA, RNA, and protein expression in vitro by multiple methods, and next generation sequencing. HPLC experience is preferred. Candidates with varying levels of experience will be considered and are encouraged to apply.
Attaching a cover letter to your application's resume files is highly encouraged - applicants can also send cover letters directly to the hiring team by reaching out to barbara.purmort@hdt.bio.
Principal Responsibilities:
- Nucleic acid manipulation methods including:
- Plasmid/construct design
- PCR and molecular cloning; transformation of e. coli
- DNA purification and preparation for RNA transcriptions
- RNA transcription and capping; downstream purification via HPLC
- RNA analysis methods including:
- SHAPE-directed RNA structure determination
- 5’/3-RACE
- Sequencing
- Gel and capillary electrophoresis
- Dot blots
- Northern blots
- Nucleic acid transfection and assays for gene transcription and translation including:
- Western blots and immunofluorescence
- qRT-PCR, in situ hybridization, nanostring
- Maintenance of multiple cell-lines, including primary cells
- Monitor laboratory supply inventories and work with purchasing agents to ensure available stock for group or area.
- Presentation of research findings at group meetings.
- Communication with research management on a regular basis.
- Maintain the highest standards of laboratory practice in line with HDT policy, including maintenance of laboratory notebook.
- Contribute freely to the team research environment in a manner conducive to the success of the research project as a whole
Qualifications:
- Excellent oral and written communication skills
- Computer literacy, proficiency in MS Office programs
- Demonstrated expertise in cloning and analysis of DNA
- Preferred experience includes:
- RNA handling and analysis
- HPLC purification of nucleic acids
- High-throughput screening of nucleic acid libraries for function in vitro
- Molecular virology
Education/Experience:
- BS/MS in a biological science with 4+/2+ years’ experience, respectively
- 2-4 years full-time laboratory experience with basic molecular cloning, PCR, and cell culture
- Background in molecular biology
This summary describes a portion of the essential functions/responsibilities of this position. It is not an exhaustive list of all job duties. Management reserves the right to modify the duties of this position as necessary.
HDT Bio is a vaccine and immunotherapy Biotechnology company with next-generation approaches designed to enable the body’s immune system to prevent and cure disease. We have engineered our technologies to Access, Activate, and Amplify the immune system’s natural ability to create antibodies and T-cells that fight cancer and infectious diseases such as SARS-CoV-2. We are developing multiple product candidates from our proprietary discovery platforms, including LION™, which has the potential to provide health benefits to a broad patient population.
HDT Bio offers a comprehensive compensation plan which includes health insurance for employees and dependents, generous PTO, stock options and retirement benefits. HDT Bio Corp. is an equal opportunity employer committed to a diverse and inclusive workforce. Qualified applicants of any race, color, religion, sex, sexual orientation, gender identity, age, national origin, veteran, or disability status are encouraged to apply. Applicants will be considered for employment without regard to these or any other actual or perceived statuses.
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