Reseach Associate I, Molecular Biology

4 weeks ago

Seattle, United States HDT Bio Full time

HDT Bio is an early-stage nucleic acid-based vaccine and therapeutics company. We are seeking an experienced research associate to work on our RNA manufacturing and quality control scientific team. A background in molecular biology, nucleic acid handling and sterile/RNase-free technique is required. The candidate’s know-how and critical thinking will help foster a culture of collaboration, innovation, and scientific excellence in our team. This individual will make an immediate impact by contributing to ongoing efforts in developing nucleic acid-based vaccines and therapeutics for COVID-19, influenza, and other infectious diseases.


Principal Responsibilities:

  • RNA preparation, including in-vitro transcription of RNA, RNA capping, RNA purification, spin-column concentration, aliquoting and inventory management.
  • RNA quality control assays – gel electrophoresis/capillary gel electrophoresis, nanodrop, dsRNA ELISA/dot blot, 5’ capping efficiency, RT-qPCR, nanoorange, qubit.
  • Cloning of novel DNA plasmids, including designing primers for Gibson assembly of DNA, screening colonies, sequence verification and glycerol stock generation.
  • DNA preparation, including spin/gravity column purification, organic extractions, ethanol/isopropanol precipitation and resuspension.
  • DNA quality control assays – diagnostic restriction digest, PCR, gel electrophoresis, nanodrop, nanoorange, qubit
  • Preparation of sterile and RNase-free solutions
  • Maintain a clean and functional workspace
  • Maintain physical inventories and LIMs
  • Monitor and purchase supplies needed to perform daily tasks in coordination with co-workers.
  • Communicate regularly with management and supervisors.
  • Work collaboratively with scientists in the team, performing tasks with minimal supervision.
  • Maintain the highest standards of laboratory practice in line with policy, including the maintenance of an electronic laboratory notebook.
  • Contribute freely to the team research environment in a manner conducive to the success of the research project as a whole.


Qualifications (Skills):

  • Proficiency in PCR, cloning, aseptic cell culture, DNase/RNase free technique and protein characterization such as SDS-PAGE/western blot is required.
  • A desire to contribute to an overall team effort and goals
  • Experience in genetics, immunology, virology, vaccinology or related research field is preferred but not necessary.
  • Excellent oral and written communication skills.
  • Computer literacy and proficiency in MS Office programs.



Education/Experience:

  • 1+ year full-time laboratory experience with molecular biology technical experience is strictly required
  • Bachelor’s degree in Molecular Biology, Immunology, Microbiology or related science field


This summary describes a portion of the essential functions/responsibilities of this position. It is not an exhaustive list of all job duties. Management reserves the right to modify the duties of this position as necessary.


HDT Bio is a vaccine and immunotherapy Biotechnology company with next-generation approaches designed to enable the body’s immune system to prevent and cure disease. We have engineered our technologies to Access, Activate, and Amplify the immune system’s natural ability to create antibodies and T-cells that fight cancer and infectious diseases such as SARS-CoV-2. We are developing multiple product candidates from our proprietary discovery platforms, including LION™, which has the potential to provide health benefits to a broad patient population.


HDT Bio offers a comprehensive compensation plan which includes health insurance for employees and dependents (inclusive of multiple plants to choose from, including a $0 premium plan with HSA and company contribution), generous PTO (11 observed holidays and week-long year-end closure), stock options with Carta Tax Advisory for equity and tax support, flexible spending accounts (FSAs), commuting benefits (ORCA or paid parking), and retirement benefits with company match.


HDT Bio Corp. is an equal opportunity employer committed to a diverse and inclusive workforce. This environment is encouraged by HDT's culture, which supports women in science through our Women+ group, creates inclusive events to highlight the diverse cultures represented by our staff, and with the adoption of an open office environment for all. Qualified applicants of any race, color, religion, sex, sexual orientation, gender identity, age, national origin, veteran, or disability status are encouraged to apply. Applicants will be considered for employment without regard to these or any other actual or perceived statuses.

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