Senior Principal Lab Technician

2 days ago


Cambridge, United States Radiant Systems Inc Full time

We are hiring Senior Principal Lab Technician Role.


Our Major Pharmaceutical Manufacturing Client is looking for Senior Principal Lab Technician in Cambridge, MA 02139 for 6+ months contract.


Please let me know if you are interested or anyone who might be interested.


Job Details:

Client: Major Pharmaceutical Manufacturing Client

Duration: 6+ months

Location: Cambridge, MA 02139

Title: Senior Principal Lab Technician


Pay Rate: $32.00 - $34.00/ Hr W2


Education: BS, MS, or PhD in relevant scientific field

Experience: If BS, then 11+ years. If MS, then 9+ years. If PhD, then 2-3 years of previous work experience.

Minimum 3 years of industry experience of hands-on NGS library generation and operating Illumina sequencing platform is required


Description:

• Establish workflows utilizing next-generation sequencing technologies to advance antibody discovery efforts from in vitro (yeast and phage), in vivo (single/bulk B cells) or de novo (AI based) platforms with the goal to more deeply interrogate repertoires, expand our knowledge of functional and sequence diversity and accelerate lead discovery.

• Working with computational scientists, establish data analysis pipelines for NGS data.

• Investigate use of NGS in other areas in Global Biologics to accelerate workflows and to enhance pipeline support and accelerate programs to IND.

• Develop and optimize NGS protocols for library generation, sequencing using Illumina MiSeq/NextSeq platforms, and library QC and analysis.

• Establish workflows using NGS in other areas in Global Biologics including cell line generation, plasmid preparation and CAR-T design characterization.

• Generate high quality NGS library (e.g. RNASeq, CITE-Seq) by rigorous optimization and QC process.

• Ability to perform DNA library fragment purification using Blue Pippin and analyze the size using Tape Station 4200.

• Library quantification using qubit and qPCR.

• Continuously improve NGS workflows with integration of automation and informatics.

• Deeply understand and troubleshoot NGS experiments to ensure assay robustness and generation of high-quality data.

• Meet established timelines, maintain accurate records of work.



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