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Scientist
1 month ago
Overview
Axle Informatics is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations around the globe. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).
Overview
Axle is seeking a highly ambitious, adaptable, structured, and detail-oriented Scientist join our vibrant team at the National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), Vaccine Research Center (VRC),Vaccine Production Program. (VPP).
Responsibilities
- Contribute to the generation of stable cell lines for vaccine and therapeutic protein candidates including monoclonal antibodies, recombinant proteins, and virus-like particles.
- Independently execute each step of the cell line development process including plasmid production, transfection, single cell cloning, clone expansion in static culture plates and shake flasks, fed-batch analysis, and clone stability studies.
- Use automated platforms for clone selection and screening.
- Prepare plasmid DNA using standard molecular cloning techniques.
- Participate in cell line development platform design, optimization, and cycle-time reduction studies including methods for clone selection, expansion, ranking, and characterization.
- Perform small-scale process development studies using various culture vessels including tissue culture plates, shake flasks, and small-scale bioreactors.
- Generate stable pools and perform transient protein production for supply of reagent-grade material.
- Provide laboratory support for medium preparation and ordering of supplies.
- Perform maintenance of cell counters and bioprocess analyzers, coordination of service visits, and regular ordering of consumables.
- Train other laboratory personnel on use of equipment and corresponding processes.
- Write and review technical reports documenting process development studies and stable cell line generation work.
- Work with other team members to generate experimental plans for process development studies.
- Write and review standard operating procedures for cell line development processes and instrumentation.
- Maintain complete documentation records, including a detailed and up-to-date laboratory notebook, to support regulatory filings.
- Regularly communicate progress to team lead and present work to cell line development team
Experience Required / Requirements
- B.S. in Biological Sciences with a minimum of 5 years of relevant work experience or M.S. in Biological Sciences with independent research experience in an academic or industrial setting.
- Hands-on experience with aseptic technique and mammalian cell culture along with supporting technologies for cell line engineering including cell counting, high-throughput transfection, single-cell cloning, and flow cytometry.
- Experience in preparing technical reports, standard operating procedures, and data presentations.
- Ability to work independently to execute defined experiments and troubleshoot issues as needed to meet project timelines.
- Ability to effectively communicate plans, results, challenges, and ideas to other team members in oral and written forms.
- Familiarity with computer software for word processing and data analysis.
- Experience in monoclonal antibody and/or vaccine production.
Benefits
- 100% Medical, Dental & Vision premium coverage for Employees
- Paid Time Off (Including Holidays)
- Employee Referral Bonus
- 401K Match
- Tuition reimbursement and professional development opportunities
- Flexible Spending Accounts:
- Healthcare (FSA)
- Parking Reimbursement Account (PRK)
- Dependent Care Assistant Program (DCAP)
- Transportation Reimbursement Account (TRN)
The diversity of Axle's employees is a tremendous asset. We are firmly committed to providing equal opportunity in all aspects of employment and will not tolerate any illegal discrimination or harassment-based age, race, gender, religion, national origin, disability, marital status, covered veteran status, sexual orientation, status with respect to public assistance, and other characteristics protected under state, federal, or local law and to deter those who aid, abet, or induce discrimination or coerce others to discriminate.
Accessibility: If you need an accommodation as part of the employment process please contact:
Disclaimer: The above description is meant to illustrate the general nature of work and level of effort being performed by individual's assigned to this position or job description. This is not restricted as a complete list of all skills, responsibilities, duties, and/or assignments required. Individuals may be required to perform duties outside of their position, job description or responsibilities as needed.
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