Sr. Associate Scientist

3 weeks ago

Seattle, United States TSR Consulting Services, Inc. Full time

80296


TSR is a premier National U.S. Staffing company with over 50 years of staffing excellence.


Our client, a leading pharmaceutical company is hiring a Sr. Associate Scientist. Please ONLY local candidates to Seattle WA.


Top Skills:

  • Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
  • Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
  • Experience working with, storing, and analyzing purified nucleic acids and proteins
  • Experience with optimizing molecular cloning reactions
  • Experience with gene editing primary human cells or immortalized cell-lines


Work Location: Seattle WA


Pay: $65-67/hour W2


Required Skills:

  • Will work in a fast-paced PD environment applying rigorous biochemical principles towards developing and characterizing ligated oligonucleotides for gene editing application
  • Ideal candidate will possess sufficient experience with handling, manipulating, and characterizing in vitro produced RNA oligomers (50 – 200 nt)
  • A successful scientist will directly employ molecular cloning, biophysical and analytical tools to develop next-generation gene edited therapies through the development of RNA oligomers
  • Design and execute nucleic acid ligation reactions using “splint ligation” methodology
  • Design and execute ion-pairing (IP) reversed-phase (RP) chromatography methods
  • Perform purification of oligonucleotides using chromatography and/or preparative PAGE
  • Design and evaluate the performance of new biophysical assays to identify and assess CQA’s
  • Manage samples and data related to gene editing platform process development
  • Provide experimental design and executional support to team members in adjacent and related functions (e.g. clinical development programs, high throughput process development, etc.)
  • Actively participate and support lab maintenance and continuous improvement initiatives
  • Maintain accurate and detailed laboratory notebook and documentation
  • Summarize and present data in group meetings and contribute to group discussions
  • Author technical reports, study memos, and protocols
  • Experience with solid-phase oligonucleotide synthesis a plus
  • Experience with bioinformatic tools used to predict RNA secondary structure a plus
  • Experience with JMP for DoE design and analysis a plus

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