Senior Associate Scientist
3 weeks ago
Job Title: Sr. Associate Scientist or Scientist, RNA Process Development
Location: Seattle, WA * 100% Onsite
Duration: 12+ Months
Pay range: $60-$68/hr on W2
Top Skills:
- Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
- Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
- Experience working with, storing, and analyzing purified nucleic acids and proteins
- Experience with optimizing molecular cloning reactions
- Experience with gene editing primary human cells or immortalized cell-lines
Description
- Seeking a highly motivated Sr. Associate Scientist, or Scientist, to work in a fast-paced PD environment applying rigorous biochemical principles towards developing and characterizing ligated oligonucleotides for gene editing applications. As a part of the Gene Delivery and Editing Process Development (GDEPD) organization, the ideal candidate will possess sufficient experience with handling, manipulating, and characterizing in vitro produced RNA oligomers (50 – 200 nt).
- A successful scientist will directly employ molecular cloning, biophysical and analytical tools to develop our next-generation gene edited therapies through the development of RNA oligomers. An outstanding candidate will be driven by a desire to create, optimize, and define processes with speed in a collaborative environment.
Key Responsibilities:
- Design and execute nucleic acid ligation reactions using “splint ligation” methodology
- Design and execute ion-pairing (IP) reversed-phase (RP) chromatography methods
- Perform purification of oligonucleotides using chromatography and/or preparative PAGE
- Design and evaluate the performance of new biophysical assays to identify and assess CQA’s
- Manage samples and data related to gene editing platform process development
- Provide experimental design and executional support to team members in adjacent and related functions (e.g. clinical development programs, high throughput process development, etc.)
- Actively participate and support lab maintenance and continuous improvement initiatives
- Maintain accurate and detailed laboratory notebook and documentation
- Summarize and present data in group meetings and contribute to group discussions
- Author technical reports, study memos, and protocols
Basic Qualifications:
- STEM degree with 2+ years of relevant industry experience; title/compensation will be commensurate with education, experience, and skillset
- Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
- Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
- Experience working with, storing, and analyzing purified nucleic acids and proteins
- Independently motivated with the ability to work in teams
- Ability to communicate clearly and concisely through oral presentation and technical writing
- Ability to leverage literature, internal and external resources to solve problems and develop new technologies
- Possess a growth mindset and the ability to thrive in a fast-paced, highly collaborative environment
Preferred Qualifications:
- Degree in biological or chemical engineering, biochemistry, or bio-related field
- Experience with optimizing molecular cloning reactions
- Experience with gene editing primary human cells or immortalized cell-lines
- Experience with solid-phase oligonucleotide synthesis
- Experience with bioinformatic tools used to predict RNA secondary structure
- Experience with JMP for DoE design and analysis
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Seattle, United States Pyramid Consulting, Inc Full timeImmediate need for a talented Senior Associate Scientist or Scientist, RNA Process Development . This is a 12+ Months contract with possible extension opportunity with long-term potential and is located in Seattle, WA (Onsite). Please review the job description below and contact me ASAP if you are interested. Job ID:24-38693 Pay Range: $60 - $68/hour....
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