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Senior Associate Scientist

2 months ago

seattle, United States SSi People Full time

Job Title: Sr. Associate Scientist or Scientist, RNA Process Development

Location: Seattle, WA * 100% Onsite

Duration: 12+ Months

Pay range: $60-$68/hr on W2


Top Skills:

  • Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
  • Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
  • Experience working with, storing, and analyzing purified nucleic acids and proteins
  • Experience with optimizing molecular cloning reactions
  • Experience with gene editing primary human cells or immortalized cell-lines


Description

  • Seeking a highly motivated Sr. Associate Scientist, or Scientist, to work in a fast-paced PD environment applying rigorous biochemical principles towards developing and characterizing ligated oligonucleotides for gene editing applications. As a part of the Gene Delivery and Editing Process Development (GDEPD) organization, the ideal candidate will possess sufficient experience with handling, manipulating, and characterizing in vitro produced RNA oligomers (50 – 200 nt).
  • A successful scientist will directly employ molecular cloning, biophysical and analytical tools to develop our next-generation gene edited therapies through the development of RNA oligomers. An outstanding candidate will be driven by a desire to create, optimize, and define processes with speed in a collaborative environment.


Key Responsibilities:

  • Design and execute nucleic acid ligation reactions using “splint ligation” methodology
  • Design and execute ion-pairing (IP) reversed-phase (RP) chromatography methods
  • Perform purification of oligonucleotides using chromatography and/or preparative PAGE
  • Design and evaluate the performance of new biophysical assays to identify and assess CQA’s
  • Manage samples and data related to gene editing platform process development
  • Provide experimental design and executional support to team members in adjacent and related functions (e.g. clinical development programs, high throughput process development, etc.)
  • Actively participate and support lab maintenance and continuous improvement initiatives
  • Maintain accurate and detailed laboratory notebook and documentation
  • Summarize and present data in group meetings and contribute to group discussions
  • Author technical reports, study memos, and protocols


Basic Qualifications:

  • STEM degree with 2+ years of relevant industry experience; title/compensation will be commensurate with education, experience, and skillset
  • Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
  • Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
  • Experience working with, storing, and analyzing purified nucleic acids and proteins
  • Independently motivated with the ability to work in teams
  • Ability to communicate clearly and concisely through oral presentation and technical writing
  • Ability to leverage literature, internal and external resources to solve problems and develop new technologies
  • Possess a growth mindset and the ability to thrive in a fast-paced, highly collaborative environment

Preferred Qualifications:

  • Degree in biological or chemical engineering, biochemistry, or bio-related field
  • Experience with optimizing molecular cloning reactions
  • Experience with gene editing primary human cells or immortalized cell-lines
  • Experience with solid-phase oligonucleotide synthesis
  • Experience with bioinformatic tools used to predict RNA secondary structure
  • Experience with JMP for DoE design and analysis