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Associate Scientist

3 months ago

Seattle, United States ECLARO Full time

Job Description: Job Title– Associate Scientist, mRNA Platform Process Development

Location: Seattle, WA * 100% Onsite

Top Skills:

• Hands-on experience with the downstream purification of biological molecules (AKTA, chromatography, TFF, viral filtration, etc.)

• Experience with molecular cloning, PAGE, and agarose gels

• Experience working with, storing, and analyzing purified nucleic acids



Seeking a highly motivated Associate, or Sr. Associate, Scientist to work in a fast-paced PD environment applying rigorous scientific principles towards developing a platform process for producing mRNA. As a part of the Gene Delivery and Editing Process Development (GDEPD) organization, the ideal Associate will possess sufficient experience in the downstream process development of biological molecules, with a high preference for any experience with developing processes with nucleic acids. The Associate will also gain familiarity with mRNA analytical methods and their interpretation in the context of mRNA attributes and function in gene editing applications.


A successful Associate will directly employ purification principles and biochemistry techniques to develop our next-generation gene edited therapies. In this capacity, the Associate will produce, purify, and characterize mRNA molecules to enable robust ex vivo studies. This candidate will use recombinant enzymes to produce and functionalize mRNA, AKTA systems to purify mRNA via affinity and other chromatography methods, TFF to further purify and formulate mRNA, as well as use HPLCs and other biochemical analytical techniques for characterization. An outstanding candidate will be driven by a desire to create, optimize, and define processes with speed in a collaborative environment.


Key Responsibilities:


• Material generation of purified mRNA

• Execute and troubleshoot IVT and downstream process development experiments

• Manage the flow of samples and data with various downstream users of mRNA

• Assist in the design of experiments and interpretation of data

• Actively participate and support lab maintenance and continuous improvement initiatives

• Maintain accurate and detailed laboratory notebook and documentation

• Summarize and present data in group meetings and contribute to group discussions

• Author technical reports, study memos, and protocols


Basic Qualifications:


• STEM degree with 2+ years of relevant industry experience; title/compensation will be commensurate with education, experience, and skillset

• Hands-on experience with the downstream purification of biological molecules (AKTA, chromatography, TFF, viral filtration, etc.)

• Experience with molecular cloning, PAGE, and agarose gels

• Experience working with, storing, and analyzing purified nucleic acids

• Independently motivated with the ability to work in teams

• Ability to communicate clearly and concisely through oral presentation and technical writing

• Ability to leverage literature, internal and external resources to solve problems and develop new technologies


Preferred Qualifications:


• Degree in biological or chemical engineering, biochemistry, or bio-related field

• Experience with JMP for DoE design and analysis

• Experience with IVT of mRNA, including successful transfer for GMP manufacturing

• Experience with recombinant nucleic acid processing enzymes

• Experience with interpreting qPCR, HPLC, NGS, and mass spec data sets

• Experience interacting and collaborating with vendors


The starting compensation for this job is a range from *** -*** The starting pay rate takes into account characteristics of the job, such as required skills and where the job is performed. Final, individual compensation and eligibility for specific benefits will be decided based on demonstrated experience and by your employer of record (and not Client).

Additional Job Requirements:

Lab w/o blood & animal

Position Handles Hazardous Materials