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Sr. Associate Scientist
2 months ago
80296
TSR is a premier National U.S. Staffing company with over 50 years of staffing excellence.
Our client, a leading pharmaceutical company is hiring a Sr. Associate Scientist. Please ONLY local candidates to Seattle WA.
Top Skills:
- Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
- Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
- Experience working with, storing, and analyzing purified nucleic acids and proteins
- Experience with optimizing molecular cloning reactions
- Experience with gene editing primary human cells or immortalized cell-lines
Work Location: Seattle WA
Pay: $65-67/hour W2
Required Skills:
- Will work in a fast-paced PD environment applying rigorous biochemical principles towards developing and characterizing ligated oligonucleotides for gene editing application
- Ideal candidate will possess sufficient experience with handling, manipulating, and characterizing in vitro produced RNA oligomers (50 – 200 nt)
- A successful scientist will directly employ molecular cloning, biophysical and analytical tools to develop next-generation gene edited therapies through the development of RNA oligomers
- Design and execute nucleic acid ligation reactions using “splint ligation” methodology
- Design and execute ion-pairing (IP) reversed-phase (RP) chromatography methods
- Perform purification of oligonucleotides using chromatography and/or preparative PAGE
- Design and evaluate the performance of new biophysical assays to identify and assess CQA’s
- Manage samples and data related to gene editing platform process development
- Provide experimental design and executional support to team members in adjacent and related functions (e.g. clinical development programs, high throughput process development, etc.)
- Actively participate and support lab maintenance and continuous improvement initiatives
- Maintain accurate and detailed laboratory notebook and documentation
- Summarize and present data in group meetings and contribute to group discussions
- Author technical reports, study memos, and protocols
- Experience with solid-phase oligonucleotide synthesis a plus
- Experience with bioinformatic tools used to predict RNA secondary structure a plus
- Experience with JMP for DoE design and analysis a plus
