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Protein Science Research Associate II
2 months ago
A-Alpha Bio is a pioneering biotechnology firm focused on advancing drug discovery through innovative methodologies. Our headquarters is located in Seattle, WA, where we are dedicated to developing impactful therapeutics by integrating high-throughput synthetic biology with cutting-edge machine learning techniques. Our collaborations with top pharmaceutical and biotech companies aim to facilitate the discovery of antibodies, molecular glues, and other therapeutics related to protein interactions.
Note: This position requires relocation to Seattle, WA.
Compensation: Competitive salary range of $73,000 - $87,000 annually.
Employment Status: Exempt
Work Environment: Onsite
Reporting Structure: Reports to the Senior Scientist, Protein Science
Role ResponsibilitiesAs a key member of our Protein Science team, you will contribute to enhancing our mammalian protein production capabilities. Your responsibilities will include:
- Designing and constructing therapeutic molecules as a molecular biologist.
- Executing mammalian cell transfections with precision and sterile techniques as a cell scientist.
- Assisting in the purification and characterization of proteins you have engineered as a protein scientist.
- Conduct molecular biology experiments and create protein expression constructs.
- Manage cell cultures for optimal protein production.
- Perform transient transfection experiments utilizing Expi293 or ExpiCHO cells.
- Prepare transfectant supernatants for subsequent protein purification.
- Maintain detailed records of protein expression activities in an electronic lab notebook and update the internal protein database.
- Engage in learning opportunities related to downstream protein characterization and functional assays.
- Present experimental findings at interdisciplinary team meetings.
- Complete additional tasks as assigned.
- Bachelor's degree in a relevant discipline with a minimum of 2 years of experience in an industrial biotechnology setting, or equivalent education and experience.
- Proficiency in Gibson Assembly and other molecular biology techniques.
- Experience in designing and ordering oligonucleotides and gene fragments.
- Familiarity with construct sequence verification.
- Understanding of protein production processes in a startup environment.
- Strong communication and organizational skills.
- Able to collaborate effectively in dynamic team settings.
- Self-driven with a passion for interdisciplinary research in a fast-paced atmosphere.
- Experience with transient protein expression at a small to medium scale.
- Familiarity with ExpiCHO and Expi293 cell culture techniques.
- Expertise in biolayer interferometry assays using Octet/Gator systems.
- Knowledge of protein biophysical characterization methods (HPLC, DSF, Bioanalyzer/SDS-PAGE, ELISA, UV280 NanoDrop, etc.).
- Experience in antibody formatting and optimizing protein expression.
We provide a competitive compensation package, including benefits, flexible time off, and equity in a rapidly growing startup. Candidates must have legal authorization to work in the United States. A-Alpha Bio is currently unable to offer visa sponsorship.
About A-Alpha BioA-Alpha Bio specializes in leveraging synthetic biology and machine learning to analyze, predict, and engineer protein-protein interactions. Our AlphaSeq platform allows for the experimental measurement of millions of protein interaction affinities simultaneously, while our AlphaBind platform uses this data to train machine learning models for predictive binding from sequence. We are committed to discovering and optimizing high-impact therapeutics both for our internal pipeline and in collaboration with leading pharmaceutical and biotechnology companies.
Founded in 2018, A-Alpha Bio emerged from the University of Washington's Institute for Protein Design and Center for Synthetic Biology, drawing on a community of innovative and entrepreneurial scientists. Our focus has been on developing an oncology-centered internal pipeline, alongside partnerships with pharma and biotech firms to collaboratively train predictive models and discover drugs for various therapeutic areas.
Our Seattle headquarters features a custom-designed lab and office space, fostering a work environment that values work-life balance. We are situated in a region known for its outdoor activities, attracting individuals with diverse interests, from outdoor enthusiasts to gamers and artists. At A-Alpha Bio, you will find a community that embraces a wide range of passions.
