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Senior Research Associate

1 month ago

Gaithersburg, Maryland, United States MESO SCALE DIAGNOSTICS, LLC. Full time
Job Summary

The Senior Research Associate in Protein Biochemistry will contribute to the development and characterization of proteins for therapeutic and diagnostic applications. This role requires a strong background in protein expression, purification, and characterization, as well as excellent analytical and problem-solving skills.

Key Responsibilities
  • Express recombinant proteins using various systems, including bacterial, mammalian, and insect cells.
  • Purify proteins using chromatographic techniques, such as affinity, ion exchange, and size exclusion.
  • Perform biochemical and biophysical assays to assess purified proteins for quality, purity, and activity, including SDS-PAGE, Western blotting, ELISA, and UV/Vis spectroscopy.
  • Analyze experimental data and present findings to the team.
  • Maintain accurate and detailed laboratory records, including experimental procedures, results, and conclusions.
  • Contribute to the preparation of technical reports, presentations, and scientific publications.
  • Participate in project meetings and contribute to the design and planning of experiments.
  • Communicate effectively with team members and external collaborators.
Requirements
  • Bachelor's degree in Biochemistry, Molecular Biology, Biotechnology, or a related field.
  • Master's degree preferred.
  • A minimum of two years of hands-on laboratory experience in protein biochemistry required.
  • Academic or industry experience preferred.
Preferred Qualifications
  • Proficiency in protein expression systems, including E. coli, HEK293, and Sf9.
  • Experience with chromatographic purification techniques and equipment, including ÄKTA systems.
  • Familiarity with protein characterization methods, including mass spectrometry, CD spectroscopy, and DSC.
  • Familiarity with methods for characterization of protein-protein interaction, including SPR, BLI, and ELISA.
Work Environment

This role will be based in a laboratory environment, working with chemical reagents and performing Biosafety Level 2 work. The successful candidate will be required to handle BSL-2 materials and organisms, including human blood and/or blood products.