Lab and Research Technician 2- Wythe Lab

3 months ago

Charlottesville, United States Commonwealth of Virginia Full time
The Wythe Lab is currently seeking a research technician to join our team to decipher the molecular mechanisms underlying the basis of vascular malformations and cardiovascular disease, with a focus on studying the vasculature in the brain. An ideal applicant will be a self started, motivated to learn new techniques, organized, works well in a team environment, and has excellent organizational skills and a good understanding of basic molecular biology or biochemistry. An added bonus is if the applicant is experienced working with cell culture models, zebrafish, or mice. This position is ideal for graduating seniors looking to take a year or two off before medical school or graduate school that would like to publish papers and get extensive research experience.

The individual hired will have a number of specific duties related to the overall goal of the research effort. They will include generating DNA constructs for generation of transgenic mice, basic manipulation of mouse embryos, maintenance of a mouse colony, and use of a variety of developmental biology, cell culture, immunohistochemical and molecular biology techniques to analyze tissues and cells in order to understand how endothelial cells differentiate into arterial and venous cells during development. The candidate will interact with the supervisor on a daily basis, but will be expected to independently perform and execute successful experiments, and to perform routine lab maintenance and organization. A lack of mouse experience can be offset by advanced skills in cell culture and biochemistry or cell-based assays, or extensive experience with zebrafish husbandry and embryology.
Total Work Effort
30%- Work with mouse or zebrafish models. General animal husbandry and maintenance of a medium size mouse or zebrafish colony. Embryo dissection and processing of fixed samples for. X-gal staining, in-situ hybridization, and antibody staining (whole mount and section) and imaging (confocal, etc.).
5%- Lab Maintenance. Sharing general lab maintenance responsibilities with other lab members (cleaning, etc), ordering supplies from vendors and negotiating price quotes, inventory of supplies and chemicals, scheduling and organizing maintenance and repair of laboratory equipment, and making general solutions.
25%- Molecular biology and biochemistry. Restriction enzyme digestion and mapping, agarose and acrylamide gel electrophoresis, isolation of DNA from agarose gel, ligation of DNA, transformation of DNA, mini- and maxi-preparation of DNA, PCR, isolation of RNA, southern, northern, and western blotting, synthesis of cDNA from RNA, quantitative RT-PCR, in-vitro transcription. Documents results of experiments (keep an electronic lab notebook and make PPTs of results).
25%- Tissue Culture. Propagation of mammalian cells, transient and stable transfection of cultured cells, isolation of DNA, RNA, and protein from cultured cells, antibody staining of cultured cells, luciferase activity quantitation in cultured cells.
10%- Data Analysis. Simple data analysis in excel spreadsheet format by performing arithmetical operations (multiplication, division, etc) on a set of data points, as well as obtaining the mean, standard deviation, and standard error of a sample set. Results would be plotted on simple charts.
5%- Performs other job related duties as assigned.

Minimum Requirements

Education: Associate's degree

Experience: At least 2 years of lab experience which may include student experience. Bachelor's degree in related science will be considered in lieu of experience.

Preferred Experience
A working, fundamental knowledge of basic molecular biology
Mouse or zebrafish husbandry and colony maintenance
PCR-based genotyping
Proficiency with the Microsoft office suite (excel, word, powerpoint) and computers in general
Rigorous attention to detail, excellent organization and record keeping skills
Familiarity with FileMaker pro
Ability to learn and employ new software for record keeping and database management.

Physical Demands

This position can spend extensive periods of time standing and squatting while conducting experiments or caring for animals. Also requires keyboarding and other bench work and animal procedures requiring finer finger manipulation.

Application Instructions: Please apply through Workday and complete an application online. Include the following documents:
A CV/resume
A cover letter

All documents can be loaded into the resume submission field, multiple documents can be submitted into this one field. Internal applicants must apply through their UVA Workday profile. Incomplete applications will not be considered. This position can not sponsors applicants who require visa sponsorship.

For questions about the application process, please contact Margaret Weeks , Academic Recruiter. Before a formal offer of employment is extended, the selected candidate will undergo a background check per university policy.

The University of Virginia, i ncluding the UVA Health System which represents the UVA Medical Center, Schools of Medicine and Nursing, UVA Physician’s Group and the Claude Moore Health Sciences Library, are fundamentally committed to the diversity of our faculty and staff. We believe diversity is excellence expressing itself through every person's perspectives and lived experiences. We are equal opportunity and affirmative action employers. All qualified applicants will receive consideration for employment without regard to age, color, disability, gender identity or expression, marital status, national or ethnic origin, political affiliation, race, religion, sex (including pregnancy), sexual orientation, veteran status, and family medical or genetic information.

  • Laboratory Research Associate 2

    2 weeks ago

    Charlottesville, Virginia, United States Commonwealth of Virginia Full time

    Position Title: Laboratory Research Associate at The Wythe LabThe Wythe Lab is actively seeking a laboratory research associate to become a vital part of our team, dedicated to exploring the molecular foundations of vascular anomalies and cardiovascular conditions, with a particular emphasis on cerebral vasculature. We are looking for an individual who is...

  • Lab and Research Technician 1- Wythe Lab

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Wythe Lab is currently seeking a research technician to join our team to decipher the molecular mechanisms underlying the basis of vascular malformations and cardiovascular disease, with a focus on studying the vasculature in the brain. An ideal applicant will be a self started, motivated to learn new techniques, organized, works well in a team...

  • Research Associate

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Wythe Lab has an open position for a postdoctoral research associate to manage research projects, train junior scientists, and help maintain a dynamic laboratory environment. We attack biological questions on multiple fronts, using gold standard genetic models-such as the mouse and zebrafish, cutting edge imaging techniques (lightsheet, micro-CT),...

  • Lab and Research Technician 1 or 2

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Winckler lab in the Department of Cell Biology, at the University of Virginia School of Medicine, is seeking a Lab and Research Technician 1 or 2 to work on experiments to understand normal and abnormal neuronal function, utilizing molecular biology, mouse genetics, primary cell culture, immunocytochemistry, and microscopy []. The incumbent will work...

  • Lab and Research Technician 2

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Zhu lab at the University of Virginia seeks a Lab & Research Technician 2 to conduct cutting-edge research in the field of neuroscience. Lab and Research Technician 2 employees provide increasingly complex technical support for projects and may engage in increasingly complex scientific experiments. May handle more sophisticated administrative and...

  • Lab and Research Technician 1-2 OR Lab Specialist, Pharmacology

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Voss Laboratory in the Department of Pharmacology at the University of Virginia is hiring a Lab and Research Technician 1-2 or a Laboratory Specialist, depending on qualifications and experience of the applicant. The Voss lab studies both clinical samples from patients with autoimmune diseases and mouse models of those disease to understand how metal...

  • Lab Technician 2

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Lukens lab (www.Lukenslab.com) in the Department of Neuroscience and the Center of Brain Immunology and Glia (BIG) at the University of Virginia is seeking to hire a motivated and detail-oriented Lab and Research Technician 2 to perform and assist with animal colony management and experimental procedures. The Lukens lab is investigating the role of...

  • Lab and Research Technician 2

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Dwyer lab in the Department of Cell Biology, at the University of Virginia School of Medicine, is seeking a Lab and Research Technician 2 to work on experiments to understand normal and abnormal brain development, utilizing molecular biology, mouse genetics, cell culture, immunocytochemistry, and microscopy. The incumbent will work closely with the PI...

  • Lab and Research Technician 2 OR Lab Specialist, Pharmacology

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Department of Pharmacology at the University of Virginia seeks either a Laboratory and Research Technician 2 or Laboratory Specialist (depending on qualifications) to assist investigators in the Bayliss Lab with experiments designed to investigate the role of transcription factors in function of brainstem respiratory neurons. The individual will...

  • Lab and Research Tech 1-2 OR Lab Specialist

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Desai lab in the Department of Pharmacology at the University of Virginia seeks a Lab and Research Tech 1-2 OR Lab Specialist. The exact position will depend on academic qualifications and lab experience. The research in Desai lab seeks to understand the mechanisms through which immune cells sense and adapt to rapid changes in the tissue environment...

  • Lab and Research Technician 2, Department of Neuroscience

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Department of Neuroscience and the Center for Brain Immunology and Glia at the University of Virginia seeks an enthusiastic laboratory technician (Lab and Research Technician II) to perform and assist with research projects and laboratory management in the Coutinho-Budd laboratory. The Technician will be responsible for assisting in research related to...

  • Lab Technician 2 Sontheimer Lab

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    Lab and Research Technician 2 employees provide increasingly complex technical support for projects and may engage in increasingly complex scientific experiments. May handle more sophisticated administrative and logistical tasks. Lab and Research Technician 2 employees operate under supervision with a degree of independence when following set protocols and...

  • Lab Technician 2 or Lab Specialist, Department of Surgery

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Department of Surgery in the School of Medicine at the University of Virginia seeks a Lab and Research Technician 2 or Lab Specialist to work in a laboratory doing translational cancer immunology research. Level will be determined by qualifications. Lab and Research Technician 2 employees provide increasingly complex technical support for projects and...

  • Lab and Research Technician

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Bao research group focuses on the membrane biology of cell communication (Bao et al., 2016, NSMB; Bao et al., 2018, Nature; Zhang et al. 2021, Nat Commun; Xiong et al., 2022 Cell). Using a combination of biochemical reconstitution, cell biology and structural approaches, we seek to elucidate how membranes and membrane proteins mediate exocytosis and cell...

  • Lab and Research Technician 2

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Biomolecular Analysis Facility (BAF) Core is seeking a Lab and Research Technician 2 to perform sample preparation, methods development, and instrument operation. The BAF Core has a Shared Instrument section (small equipment) which this position will also maintain for open access. The Core processes samples for proteomics and metabolomics by mass...

  • BIOL - Pani Lab and Research Tech 2

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Pani Lab in the Department of Biology at the University of Virginia seeks applications for two Lab Research Technician 2 positions in the area of cell biology and regeneration. The Pani lab uses acoelomorph flatworms and C. elegans to investigate the cellular and mechanisms underlying development, homeostasis, and regeneration. The two open Research...

  • Lab Technician 2, Radiation Oncology

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Department of Radiation Oncology at the University of Virginia School of Medicine is seeking a Lab Technician 2 to work in the lab of Dr. Wenpeng Liu. Lab and Research Technician 2 employees provide increasingly complex technical support for projects and may engage in increasingly complex scientific experiments. May handle more sophisticated...

  • Lab Technician 2

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Cliffe Lab in the Department of Microbiology, Immunology and Cancer Biology, at the University of Virginia School of Medicine, is seeking a Laboratory Technician level 2 to work on projects investigating Herpes Simplex Virus (HSV) infection in neurons and activation of innate sensing pathways. The lab technician will work closely with the PI to use...

  • Lab and Research Technician

    2 days ago

    Charlottesville, Virginia, United States University of Virginia Full time

    About the PositionThe Department of Pediatrics at the University of Virginia's School of Medicine is seeking a highly skilled and motivated individual to fill the role of Lab and Research Technician. This position will be responsible for carrying out research studies and assisting in experiments for the Infectious Diseases Division in the laboratory of Prof....

  • Lab and Research Technician 1

    3 months ago

    Charlottesville, United States Commonwealth of Virginia Full time

    The Flowers lab (www.flowerslab.org) in the Department of Neuroscience at the University of Virginia is seeking to hire two motivated and detail-oriented Lab and Research Technicians to perform and assist with animal colony management, lab management, and experimental procedures. The Flowers Lab is focused on Alzheimer’s disease with an interest in APOE,...