Senior Scientist: ELISA, PCR, Cell Culture

3 weeks ago


Rockville, Maryland, United States Thermo Fisher Scientific Full time

Task 1: Support lead scientist to deliver top candidates of site-specific integration (SSI) platform in the Client's Vx CHO cells through a genome-wide screening approachTask 2: Support lead scientist to reverse-engineer pre-existing high-producing CHO clones into PoC SSI candidatesPlasmid and viral vector cloning, qPCR/ ddPCR, wet lab next-generation sequencing (NGS)Experience with CRISPR or other genomic editing toolsApplies detailed fundamental knowledge of own specialty area or scientific discipline into solving process development problemsBachelor's degree in molecular biology, cell biology, virology, or other vaccinology-related discipline or equivalent and relevant formal academic / vocational qualificationHands-on experience with aseptic small scale (plate- to shake flask-based) mammalian cell culture (thawing, expanding, freezing), transfection techniques, and clonal isolationHands-on experience with common nucleic acid- and protein-based in-process analytic assays (e.g., PCR, RTPCR, ELISA, SDS-PAGE, Western blot)



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