Scientist, Protein Science

4 weeks ago


Rockville, United States Hansoh Bio Full time

**HANSOH BIO**

**BIOTHERAPEUTICS - MARYLAND R&D CENTER**

**THE POSITION - SCIENTIST / SENIOR SCIENTIST**

**RESPONSIBILITIES**
- Establish and develop efficient molecular biology capabilities to generate DNA constructs for transient mammalian cell expression of antibodies and proteins to support therapeutic discovery and engineering efforts
- Build consistent and robust protein expression capabilities for transient 293 and CHO cells that encompass HTP small scale through gram level protein production
- Perform antibody characterization utilizing ELISA, Octet or Biacore
- Perform _in vitro_ cell-based experiments using standard immunological, cellular and molecular biology techniques such as Western Blots, PCR/qPCR, ELISA, Luminex, flow cytometry, spectroscopy, and microscopy
- Design key scientific experiments together with project teams
- Desire and ability to learn new skills to help contribute to our multi-disciplinary teams
- Provide timely communication and presentations to project team and governance body
- Assist with general lab management and upkeep including maintenance of biological samples, lab supplies, biological and chemical wastes, and calibration of instruments, in accordance with established guidelines
- Diligent maintenance of lab records and logs in accordance with the standard practices
- Other related research functions as deemed necessary to support day-to-day functioning of the lab

**QUALIFICATIONS**
- PhD. degree in chemical biology, molecular biology, cell biology or equivalent scientific discipline from an accredited college or university with mínimal 2 years of relevant experience
- B.S. or M.S. degree in chemical biology, molecular biology, cell biology or equivalent scientific discipline from an accredited college or university with 10 years (with B.S.) or 5 years (with M.S.) of hands-on laboratory experience in an industrial or academic setting
- Experience with various _in vitro_ and cell-based assay, mammalian cell culture, maintenance of cell lines, transfection, cell-based functional assays, flow cytometry, ELISA, Western Blots and PCR/qPCR
- Experience with antibody characterization (LC-MS, HPSEC, Octet, Biacore) highly desired
- Experience with _ÄKTA_ purification system or iQue3 preferred
- Familiar with GraphPad Prism software
- Experience in cloning of antibody into expression vector, display technology, antibody humanization and affinity optimization preferred
- Proficient in computers with excellent record keeping, documentation and good organizational skills
- Professional verbal and written communication and interpersonal skills, including ability to interpret and understand SOPs and laboratory procedures
- Detail-oriented, analytical with excellent trouble shooting skills
- Flexible, forward thinking and motivated with ability to act independently

Pay: $95,000.00 - $115,000.00 per year

**Experience**:

- antibody characterization (LC-MS, HPSEC, Octet, Biacore): 2 years (required)
- cloning of antibody into expression vector: 2 years (required)
- antibody humanization and affinity optimization: 1 year (required)

Ability to Commute:

- Rockville, MD 20850 (required)

Ability to Relocate:

- Rockville, MD 20850: Relocate before starting work (preferred)

Work Location: In person



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