Research Associate

5 days ago


Berkeley, United States Aikium Inc. Full time

Are you a driven researcher with a passion for protein sciences, eager to make your mark in an innovative pre-seed stage TechBio startup?


Aikium Inc. is developing a proprietary technology poised to revolutionize protein therapeutics. We are seeking motivated individuals ready to grow with us, contribute to cutting-edge research, and work with an exceptional team in a collaborative and dynamic environment. You'll work on exciting projects involving biochemistry, molecular biology, protein expression, and purification.


Key qualities we value: optimism, attention to detail, and the ability to follow instructions and establish SOPs. We offer competitive pay and generous equity for the right candidate. Join Aikium as we shift the paradigm in protein engineering


This is a 40-hour / week contractor position with the potential to convert to full-time in ~6 months.


Responsibilities:

  • Clone and sub-clone genes into plasmid vectors
  • Prepare plasmid mini-prep/PCR reactions for sequencing
  • Prepare  buffers and reagents  for protein purification
  • Express challenging proteins in E. coli
  • Purify proteins using chromatography, including affinity, ion exchange, and size exclusion (inclusion body purification experience is a plus)
  • Analyze proteins using SDS-PAGE, western blots, and UV-Vis spectroscopy
  • Investigate protein-protein interactions using ELISA and other fluorescence-based methods


Requirements:

  • Bachelor’s Degree in biological sciences with 1-2 years of research experience (in industry or during Masters)
  • Hands-on experience with molecular biology techniques including PCR, DNA isolation, cloning including Gibson assembly, Golden gate cloning, Gateway cloning, etc.
  • Experience with E. coli expression of recombinant proteins and affinity-based purification
  • Experience with fluorescent plate-based assays (e.g., fluorescence polarization, anisotropy, FRET) is a plus
  • Ability to maintain accurate and detailed records
  • Ability to work with moderate to minimal supervision

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