NGS Scientist

3 weeks ago


Cambridge, United States Randstad Life Sciences US Full time

Next-Generation Sequencing (NGS) Scientist

5 Months

Cambridge, MA


Job Responsibilities

  • Establish workflows utilizing next-generation sequencing technologies to advance antibody discovery efforts from in vitro (yeast and phage), in vivo (single/bulk B cells) or de novo (AI based) platforms with the goal to more deeply interrogate repertoires, expand our knowledge of functional and sequence diversity and accelerate lead discovery.
  • Working with computational scientists, establish data analysis pipelines for NGS data.
  • Investigate use of NGS in other areas in Global Biologics to accelerate workflows and to enhance pipeline support and accelerate programs to IND.
  • Develop and optimize NGS protocols for library generation, sequencing using Illumina MiSeq/NextSeq platforms, and library QC and analysis.
  • Establish workflows using NGS in other areas in Global Biologics including cell line generation, plasmid preparation and CAR-T design characterization.
  • Generate high quality NGS library (e.g. RNASeq, CITE-Seq) by rigorous optimization and QC process.
  • Ability to perform DNA library fragment purification using Blue Pippin and analyze the size using Tape Station 4200.
  • Library quantification using qubit and qPCR.
  • Continuously improve NGS workflows with integration of automation and informatics.
  • Deeply understand and troubleshoot NGS experiments to ensure assay robustness and generation of high-quality data.
  • Meet established timelines, maintain accurate records of work.

Education & Qualifications

  • Education: BS, MS, or PhD in relevant scientific field
  • Experience: If BS, then 11+ years. If MS, then 9+ years. If PhD, then 2-3 years of previous work experience.
  • Minimum 3 years of industry experience of hands-on NGS library generation and operating Illumina sequencing platform is required

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