Senior Cellular Genomics Specialist
3 days ago
Senior Cellular Genomics Specialist
Location:
Cambridge, MA
Type:
Contract
Responsibilities:
Execute established lab protocols to:
• Participate in sample collection of murine and human tissues, including preparation of tubes and interfacing with in vivo scientists to retrieve samples.
• Isolate primary immune cells and perform standard mammalian cell culture.
• Prepare murine and human tissues for sample preparation, including tissue fixation, dissociation, sectioning, and antibody staining.
• Generate spatial and single-cell data via commercial workflows, including 10x Genomics and Parse Technologies.
• Interpret quality control metrics for spatial, single-cell, and cytometry experiments.
Requirements:
• Master's degree in Biology, Immunology, or a related field, plus 3 years of experience.
• 3+ years of hands-on experience with standard immunoprofiling technologies, including Flow Cytometry.
• Single-cell library preparation (ideally 10x Genomics or Parse Technology).
• Preparation and loading of Illumina sequencing libraries.
Bonus:
• Generation of spatial transcriptomics data (any technology).
• 3+ years of experience with primary immune cells, including primary immune cell isolation, maintenance of immune cell lines, cell culture, and in vitro stimulation of primary immune cells.
Bonus:
• Experience with CRISPR-mediated functional genomics, including electroporation of primary immune cells.
Benefits:
System One offers eligible employees health and welfare benefits coverage options, including medical, dental, vision, spending accounts, life insurance, voluntary plans, and participation in a 401(k) plan.
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