Laboratory Assistant

3 weeks ago

San Diego, United States UC San Diego Health Full time

Payroll Title:

- SRA 1
- Department:

- CANCER CENTER/Solid Tumor Therapeutics
- Hiring Pay Scale
- $25.75 - $30.72 / Hour
- Worksite:

- Moores Cancer Center
- Appointment Type:

- Career
- Appointment Percent:

- 100%
- Union:

- RX Contract
- Total Openings:

- 1
- Work Schedule:

- Days, 8 hrs/day, Monday-Friday**#129668 Laboratory Assistant***:Extended Deadline: Tue 5/21/2024**

**UC San Diego values equity, diversity, and inclusion. If you are interested in being part of our team, possess the needed licensure and certifications, and feel that you have most of the qualifications and/or transferable skills for a job opening, we strongly encourage you to apply.**

**DESCRIPTION**:
The Moores Cancer Center (MCC) is one of just 55 NCI-designated Comprehensive Cancer Centers in the United States and the only one in San Diego County. As a consortium cancer center, it is a collaborative partnership between the UCSD: encompassing 28 departments, 6 schools (School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, School of Public Health, Jacobs School of Engineering, School of Biological Sciences & the School of Physical Sciences), UCSD Health oncology hospitals and clinics; the basic and public health research and outreach of San Diego State University (SDSU), and the basic and translational research of the La Jolla Institute of Immunology (LJI). These various programs and units are all dedicated to fulfilling the Moores Cancer Center's mission of reducing cancer's burden. As such, it ranks among the top centers in the nation conducting the continuum of cancer research, providing advanced patient care, and serving the community through outreach and education programs. As a top-ranking, future-oriented organization, we offer challenging career opportunities in a fast-paced and innovative environment. Moores Cancer Center follows a progressive philosophy of career-path development for its employees including opportunities for cross-training, professional development, and progressive responsibility.

MCC's mission is to transform cancer care in our catchment area and beyond by driving exceptional scientific discoveries and innovations in prevention, detection, care, and training. MCC will make a global impact on improving health by reducing cancer burden through accelerated discovery and translation, compassionate and interdisciplinary care, education and community engagement, with the foundation of our core values - Excellence, Innovation and Risk-Taking, Collaboration, Diversity and Service.

The pancreatic cancer program at Moores Comprehensive Cancer Center at UC San Diego has several projects that investigate specific aspects of pancreatic cancer biology and focus on developing new, more effective therapies. Within Dr. Simeone's lab, we generate and utilize various cancer models, including genetically engineered mouse models (GEMMs), patient-derived xenografts, primary tumor organoids, and patient-derived cell lines to study pancreatic cancer genetics, development, and chemoresistance.

Under supervision, assist with the characterization of "POLQ Synthetic Lethality in HR-Deficient Pancreatic Adenocarcinoma" and preclinical studies. Assist with recombinant DNA work and genome engineering. Assist with various cell culture techniques such as performing flow cytometry analysis of cell surface protein expression, cell propagation, contaminant testing, and cryopreservation. Assist with mouse colony management and genotyping using established PCR techniques. Work with small animals and assist with procedures such as surgery, pancreatic orthotopic injections, ultrasound imaging, and monitoring pregnancies. Assist with immunohistochemistry of frozen and fixed tissues or cells. Assist with protein biochemistry techniques including protein quantification, SDS PAGE, immunoprecipitation, and Western blotting. Maintain and prepare accurate records of all experiments. Assist in the preparation of data for publication. Other duties assigned as needed.

**MINIMUM QUALIFICATIONS**:

- Theoretical knowledge of molecular biology, immunology, cell biology, and/or biochemistry.
- Ability to perform molecular biology techniques including PCR, RT-PCR, cDNA synthesis, gene expression analysis, recombinant protein expression, RNA/DNA isolation, protein purification.
- Ability to perform cell biology techniques including: mammalian cell culture, DNA transfection, cell fractionation and differential centrifugation, fluorescence
- and luminescence-based bioassays.
- Ability and willingness to handle and work with small animals. Experience to assist with mouse genotyping using DNA isolation, established PCR-based methods and argarose gel electrophoresis. Ability to assist in performing mouse surgeries, experiments, and injections.
- Ability to perform biochemical assays including protein quantification using BCA, running SDS-PAGE gel, immunoblot and co-immunoprecipitation.
- Ability to perform DNA and protein characte

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