Research Technician

2 weeks ago

Boston, United States Massachusetts General Hospital Full time

GENERAL SUMMARY/ OVERVIEW STATEMENT:

Under the direct supervision of the Principal Investigators in the Center for Engineering in Medicine the Research Technician will perform work to support a laboratory's research endeavors in the laboratory. The laboratory performs isolation, multiplexed cellular and molecular analysis of cells in various tissues. The responsibilities include collection of and accurate recording of procedures and results, ordering necessary reagents to conduct experiments, and participation in joint upkeep of the lab.

PRINCIPAL DUTIES AND RESPONSIBILITIES:

* Carries out a wide range of lab techniques and properly follows protocols and instruction. These may include culture of primary human and animal cells and cell lines, molecular biology, PCR, ddPCR, performing microfluidic isolation of cells, testing, flow cytometry, sample preparation for RNA sequencing. Applies knowledge of molecular biology, biochemistry and/or bioengineering in the performance of research investigation.
* Correctly operates equipment used within the research laboratory. Reports equipment malfunctions to management. Provides assistance with maintenance and monitoring of equipment.
* Collects and records study data. Synthesizes and interprets information obtained from graphs, charts, laboratory experiments, statistical summaries and other data collection methods and provides to PI. Effectively uses computer programs as well as performs data entry into databases.
* Maintains a lab notebook (physical and electronic). Prepares written progress reports for PI on status of research, technical procedures, and sequence of operations; includes statistical and graphic summaries of findings and possible conclusions. Assists the Investigator with the preparation of reports and papers for publication, such as data analysis and preparation of charts, and may be included as an author.
* Participates in the upkeep of the lab, keeping both direct and general workspaces clean and tidy; helps to maintain an overall safe and clean environment.
* Prepares stocks of necessary reagents and maintains laboratory supplies.
* Works safely within the lab environment and in performing work duties. Wears personal protective equipment as required.
* Notifies and seeks assistance from senior lab personnel or supervisor for any questions, unusual experimental conditions or results or needed training.
* Aids with a range of administrative tasks to include supply ordering and mail or package pick up and distribution.
* Attends lab meetings and journal clubs. Periodically presents in the meetings as assigned by the PI.
* Performs other duties as assigned.

SKILLS/ABILITIES/COMPETENCIES REQUIRED:

* Scientific and research knowledge relevant to techniques and equipment used in a research lab. These include knowledge of molecular and cellular biology and skill in PCR, qPCR, protein analysis and mammalian cell culture. Additional skills/classes in sequencing, mass spectrometry fundamentals and R programming language are considered pluses.
* Ability to learn new techniques, including microfluidics, cytometric methods as well as specialized software for data analysis.
* Must be self-motivated and able to work independently as well as part of a team. Willing to learn and motivation are highly critical for this position.
* Ability to work carefully, accurate and consistent with instruction or protocol.
* Must be able to work safely and properly use protective equipment.
* Excellent verbal and written communication skills.
* Proficient computer skills including Word, PowerPoint, Excel and knowledge of statistical programs.
* Is knowledgeable and compliant in all hospital, State and Federal regulatory requirements, including hospital policy and procedures (where applicable to the performance of the job), Joint Commission on Accreditation of Healthcare Organizations (JCAHO), and Health Insurance Portability and Accountability Act of 1996 (HIPAA).

EDUCATION:

Bachelor's degree in biological or physical sciences.

EXPERIENCE:

None. Related experience as obtained through a baccalaureate degree program and to include molecular biological, analytical techniques. Experience with microfluidics, flow cytometry, mass spectrometry, RNAseq, cell culture are pluses.

WORKING CONDITIONS:

* Duties will be performed in a research laboratory. This setting may create contact or exposure to one or more of the following: chemicals, fluids, sharp instruments and other supplies and equipment consistent with a research lab. Contact or exposure may be airborne or physical.
* Work will be conducted over a forty-hour work week.

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