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Senior Researcher

1 month ago

Bothell, United States Mindlance Full time

Job Title: Senior Research Associate

Location: Bothell, WA

Duration: 8+ months with possible extension


Summary

Seagen Research is seeking a collaborative and independent Scientist to participate in development of antibody-based oncology therapeutics. The Scientist will report to the Molecular and Cellular Technologies team lead within Antibody Discovery. Their primary responsibilities will include engineering cell lines and performing flow-based assays to support antibody campaigns. The successful candidate will work well in a highly collaborative team environment and possess expertise in flow cytometry, mammalian cell culture, and cell engineering.


Responsibilities

• Design, execute, troubleshoot, optimize, and interpret experiments • Order constructs for overexpression and knockout cell line generation • Engineer cell lines using transfection, nucleofection, CRISPR/Cas9, lentiviral transduction, and other methodologies • Evaluate target expression on engineered and endogenous cell lines using flow cytometry and molecular characterization • Perform routine cell culture maintenance and banking, working with both primary and cancer cells • Optimize and perform flow cytometry assays to screen and characterize antibodies • Maintain laboratory spaces, equipment, and supplies • Keep an up-to-date and accurate electronic laboratory notebook • Present experimental findings and contribute to scientific discussion at group and cross-functional team meetings • Proactively seek guidance and build knowledge relevant to the role


Required Qualifications

• Bachelor’s degree + 4 years laboratory research experience or Master’s degree + 2 years laboratory research experience in biological sciences or related subject • Proficiency in culturing/maintaining multiple cell lines under BSL2 conditions • Knowledge of cell engineering platforms, including transient transfection, generation of stable cell lines, and CRISPR • Experience in multi-color flow cytometry • Demonstrated ability to work independently and collaboratively, according to project needs • Strong written and verbal communication skills, including ability to maintain a good working laboratory notebook


Preferred Qualifications

• Experience with FACS and single-cell cloning • Basic molecular biology skills, including vector design, cloning, and PCR • Knowledge of antibodies or antibody drug conjugates (ADCs).


EEO:

Mindlance is an Equal Opportunity Employer and does not discriminate in employment on the basis of Minority/Gender/Disability/Religion/LGBTQI/Age/Veterans.