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Senior Associate Scientist
2 months ago
Associate Director, Gene Therapy Research
Working within Fractyl Health's Gene Therapy Research Team, the Senior Associate Scientist will be integral to discovering and developing the next generation of gene therapies centered on treating unmet medical needs due to metabolic disease. Successful candidates will be responsible for contributing to Fractyl's pipeline gene therapy programs with a primary focus on developing and testing therapies for the treatment of diabetes. As a Senior Associate Scientist - Molecular Biology, you will participate primarily in the generation and evaluation of novel transgene constructs, and the development and application of cell-based in vitro assays to evaluate performance of the constructs. You will be a part of an exciting and dynamic environment where these responsibilities and roles may shift as we advance our Gene Therapies to the clinic, providing new opportunities for enriching your professional skillset.
Maintain and culture cells (e.g., HEK293, immortalized endocrine cells, primary cells)
Screen transgene construct expression and performance in transfected/transduced cells
Development and application of molecular and cell-based assays (e.g., gel electrophoresis, PCR, qPCR, ddPCR, ELISA, western blot/Jess, flow cytometry, MSD)
Assist in AAV production, purification, and QC as needed
Apply molecular/biochemical assays to and studies as needed
Analyze experimental data, generating plots and summarizing results and conclusions
Maintain a comprehensive Electronic Lab Notebook documenting experiments
Perform all activities in compliance with applicable regulations, Fractyl's policies and guidelines, including all required applicable training
Bring a "can do" spirit to work and deliver on other responsibilities as assigned
Applicable experience in biotech, pharmaceutical or medical device industries preferred
Prior vocational experience in related fields a plus (i.e. Co-Ops, Internships, Fellowships, etc.)
Oligo and plasmid design using software (e.g., SnapGene, Benchling)
PCR amplification, gel electrophoresis and analysis
DNA restriction and ligation (e.g., restriction enzymes, Golden Gate, Gibson)
Bacterial transformation, plating, culturing, DNA prep, and colony screening
Submission of samples for Sanger sequencing and sequencing analysis
Experience in DNA, RNA, and protein isolation from cells and/or tissue samples
Experience in nucleic acids handling, quantitation, and analysis (e.g. Nanodrop, Qubit, Bioanalyzer qPCR, ddPCR, RT, etc.)
Experience in protein analysis (e.g., ELISA, Western blot, Jess, etc.)
Experience in cell-based assays (e.g. flow cytometry, luminescence assays, etc)
Experience in cell culture work (e.g., maintenance, transfection, transduction)
Experience in AAV production (Preferred)
Experience with electronic lab notebook documentation (Preferred)
Demonstrated record of intellectual curiosity, innovation and creative problem solving with an entrepreneurial spirit
Ability to lead fast-paced projects with a keen sense of urgency to get the job done well
Evidence of "hands-on" experience and expertise
Proven and successful track record as a team-player and collaborator in small working environments
Highly organized and detail oriented with a passion to deliver quality results
Excellent verbal and written communication skills, with experience translating technical concepts into user-friendly documentation
Highest levels of professionalism, confidence, personal values and ethical standards
