Research Associate
2 weeks ago
This position will remain open until a successful candidate has been identified.
UCSD Layoff from Career Appointment : Apply by 03/15/2024 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants : Apply by 03/26/2024. Eligible Special Selection clients should contact their Disability Counselor for assistance.
DESCRIPTIONThe Moores Cancer Center (MCC) is one of just 55 NCI-designated Comprehensive Cancer Centers in the United States and the only one in San Diego County. As a consortium cancer center, it is a collaborative partnership between the UCSD: encompassing 28 departments, 6 schools (School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, School of Public Health, Jacobs School of Engineering, School of Biological Sciences & the School of Physical Sciences), UCSD Health oncology hospitals and clinics; the basic and public health research and outreach of San Diego State University (SDSU), and the basic and translational research of the La Jolla Institute of Immunology (LJI). These various programs and units are all dedicated to fulfilling the Moores Cancer Center's mission of reducing cancer's burden. As such, it ranks among the top centers in the nation conducting the continuum of cancer research, providing advanced patient care, and serving the community through outreach and education programs. As a top-ranking, future-oriented organization, we offer challenging career opportunities in a fast-paced and innovative environment. Moores Cancer Center follows a progressive philosophy of career-path development for its employees including opportunities for cross-training, professional development, and progressive responsibility.
MCC's mission is to transform cancer care in our catchment area and beyond by driving exceptional scientific discoveries and innovations in prevention, detection, care, and training. MCC will make a global impact on improving health by reducing cancer burden through accelerated discovery and translation, compassionate and interdisciplinary care, education and community engagement, with the foundation of our core values - Excellence, Innovation and Risk-Taking, Collaboration, Diversity and Service.
Perform tumor immunology studies consisting of murine models of cancer, animal breeding and genotyping, flow cytometry, single cell sequencing, immunohistochemistry, in vitro cell biology, and in vitro biochemistry. Murine tumor biology studies will include xenograft, orthotopic and spontaneous tumor growth and metastasis assays, immunological analysis, treatment of animals with experimental therapeutic agents by gavage, intravenous and intraperitoneal methods, breeding and genotyping of transgenic and mutant mice, and immune cell isolations. Perform major surgery, including small animal anesthesia. In addition, perform immunohistochemistry of frozen and fixed tissues or cells. Conduct various in vitro cell biology techniques to include performing flow cytometry analysis of cell surface protein expression, cell adhesion and migration assays, transfecting cells with expression constructs, preparing lysates of cells in culture or tissues, quantification, ELISA assays, SDS PAGE, Western and Immunoblotting and gel electrophoresis. Additionally, perform recombinant DNA techniques to include preparing plasmids and maintaining plasmid stocks, analyzing DNA and RNA purification, and conducting DNA electrophoresis, and RT-PCR. Make innovative contributions regarding experimental design and methodology, using published scientific data in referred journals, as well as online using search engines and subject-specific websites, to make recommended changes to current protocols and practices. Troubleshoot problems for quality control on immunochemical and histochemical procedures. Maintain and prepare accurate records of all experiments. Assist in the preparation of data for publication. Assume responsibility for lab maintenance and safety training. Provide training and work direction in areas of expertise as needed. Maintain and prepare accurate records of all experiments. Assist in the preparation of data for publication. Other duties assigned as needed.
MINIMUM QUALIFICATIONS- Advanced theoretical knowledge of molecular biology, biochemistry, physiology and cell biology or a Bachelor's of Science degree in an appropriated field of science or equivalent in education and experience.
- Experience independently isolating bone marrow and blood cells for analysis by flow cytometry, immunoblotting, gene expression and cell adhesion assays.
- Experience independently performing and directing in vivo experiments including the injection of growth factors, tumor cells and therapeutics, bleeding and
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