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Scientist
3 months ago
The Scientist Cell Sourcing will lead the development, implementation, and refinement of robust protocols for the isolation and expansion of primary liver and kidney cells.
This role, and team, will be tasked with developing large scale, GMP level protocols to supply cells for R&D cellularization efforts in decellularized liver and kidney scaffolds, and with providing necessary data and documentation support for the preparation of regulatory filings.
- Coordinate with R&D and manufacturing teams to generate technical plans to develop and optimize targeted primary cell isolation protocols from human liver and kidney tissue
- Draft protocols, experimental documentation strategies, summaries, and reports
- Participate in drafting of documentation for regulatory submissions
- Work with a team on a flexible schedule, according to organ availability, to conduct primary cell isolation protocols
- Design and execute experiments to develop methods for downstream purification and expansion of primary liver and kidney cells
- Work with manufacturing and engineering teams to design methods of control for the generation of reliable primary cell manufacturing processes that will adhere to GMP standards
- Perform characterization of cells obtained from isolation and expansion protocols using standard techniques such as flow cytometry and IHC
- Assist in coordination and training activities for tech transfer of fully developed protocols to manufacturing teams
- Communicate using both oral presentations and written documents to broad level of audiences including R&D teams, quality, manufacturing, and upper management
- Contribute to efforts to understand, explore, and incorporate new technologies to advance programs
- Author and submit manuscripts, grants, and abstracts with approval of management
Skills:
Additional Skills & Qualifications:
Doctor of Philosophy (PhD) in Tissue Engineering, Biomedical Engineering, Biological Sciences, or related field
2+ of relevant experience working with primary mammalian cell isolation and culture
1+ years of relevant industry experience working in a quality-controlled research environment
Experience with primary cell isolations (required Sr
Knowledge of liver and/or kidney function and experience working with cells from these organs (required Sr
Experience working with human tissue
nowledge of perfusion control and fluid flow dynamics
Experience with cell purification techniques such as MACS sorting or size exclusion
Experience with flow cytometry
Experience with immunohistochemistry or immunofluorescence staining
About Actalent
Actalent is a global leader in engineering and sciences services and talent solutions.
We help visionary companies advance their engineering and science initiatives through access to specialized experts who drive scale, innovation and speed to market.
With a network of almost 30,000 consultants and more than 4,500 clients across the U.S., Canada, Asia and Europe, Actalent serves many of the Fortune 500.
Diversity, Equity & InclusionAt Actalent, diversity and inclusion are a bridge towards the equity and success of our people.
DE&I are embedded into our culture through:
- Hiring diverse talent
- Maintaining an inclusive environment through persistent self-reflection
- Building a culture of care, engagement, and recognition with clear outcomes
- Ensuring growth opportunities for our people
The company is an equal opportunity employer and will consider all applications without regard to race, sex, age, color, religion, national origin, veteran status, disability, sexual orientation, gender identity, genetic information or any characteristic protected by law.
