Single-Cell / Spatial Profiling Specialist

Found in: Appcast US C2 - 1 week ago

North Chicago, United States US Tech Solutions Full time

Position Title: Single-Cell / Spatial Profiling Specialist

Location: North Chicago IL 60064

Contract: 09+ months with possible extension


Key Responsibilities:

• Reporting to the Single Cell Lead, work closely with other NGS Technical Leads and staff to fulfill single cell/spatial transcriptomic profiling needs for active studies from client Genomic Research Center (GRC) stakeholders and client therapeutic area (TA) scientists

• Support single cell and spatial transcriptomic workflows. Perform sample and library prep, and sequencing, initial data QC and first pass analysis of results. Closely work with NGS technical leads and partner with bioinformatics specialists and disease area scientists to interpret results and troubleshooting.

• Operate and maintain NGS and its supportive platforms within centralized technology group. Prepare written summaries of experimental results and verbally present at team meetings.

• Maintain accurate documentation of experiments, protocols, and results. Support inventory and maintenance.

• Effectively communicate with stakeholders, especially around experimental design, troubleshooting, and responding to changes in priorities and adjusting timelines.


Qualifications:

• Bachelor’s Degree or equivalent education and typically 7 years of experience, Master’s Degree or equivalent education and typically 5 years of experience or PhD with 0-2 years experience in molecular biology, genetics, genomics or a related field

• Demonstrated record of high-proficiency, hands-on molecular biology experience with PCR-based assays and technical knowledge of high-throughput genomic profiling technologies with emphasis on Illumina sequencing platforms and complex library preparation workflows for bulkRNA-seq, 10X Genomics scRNA-seq, and 10X Genomics Visium spatial transcriptomics

• Knowledge of QC metrics, interpretation, and demonstrated use of approaches for assessing the quality of samples and libraries for bulkRNA, scRNA, and spatial profiling, such as capillary electrophoresis, qPCR, fluorometry, spectrophotometry, cell counting and viability

• Proficiency with basic bioinformatics tools and sequencing data analysis concepts, such as Illumina SAV, bcl2fastq/BCLConvert, fastQC, and working in a UNIX HPC environment. Experience with 10X Genomics Cellranger and/or Spaceranger software is highly preferred.

• Experience being part of a centralized high-throughput genomic lab is highly desirable. Industry experience is preferred but willing to consider individuals with equivalent years of experience in academic or non-industry research core lab settings.

• High attention to detail, self-motivated, critical thinking, strong written and verbal communication skills

• Ability to work in fast-paced, dynamic environment both collaboratively and independently

• Proven direct end-to-end hands-on experience with 10X Genomics technologies such as single-cell RNAseq or Visium is highly preferred.

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