Next Generation Sequencing Scientist

1 month ago

North Chicago, United States Collabera Full time
Job DescriptionJob Description

·         We supports preclinical and clinical research teams by applying genomic profiling technologies with the goal of improving the success rates for drug discovery and development.

·         We apply genomic profiling technologies to identify and validate targets and biomarkers, improve understanding of preclinical models and candidate drug molecules, select/stratify patients and understand patient responses in clinical trials.

·         We are seeking to hire a scientist proficient in Next-Generation-Sequencing (NGS) to support single cell and Visium spatial transcriptomic profiling capabilities.

·         The successful candidate will work closely with NGS technical leads and be responsible for carrying out end-to-end NGS experiments and communicating results to stakeholders and collaborators.

·         This role is expected to be on-site and in the lab full time unless otherwise approved by the supervisor.

 

What are the top 3-5 skills, experience or education required for this position?

·         Hands-on single cell sequencing experience

·         Hands-on spatial transcriptomics experience

·         NGS data QC experience

·         Hands-on NGS experience

·         Molecular biology/NGS technical knowledge

 

Key Responsibilities:

·         Reporting to the Single Cell Lead, work closely with other NGS Technical Leads and staff to fulfill single cell/spatial transcriptomic profiling needs for active studies from Genomic Research Center (GRC) stakeholders and therapeutic area (TA) scientists

·         Support single cell and spatial transcriptomic workflows. Perform sample and library prep, and sequencing, initial data QC and first pass analysis of results.

·         Closely work with NGS technical leads and partner with bioinformatics specialists and disease area scientists to interpret results and troubleshooting.

·         Operate and maintain NGS and its supportive platforms within centralized technology group. Prepare written summaries of experimental results and verbally present at team meetings.

·         Maintain accurate documentation of experiments, protocols, and results.

·         Support inventory and maintenance.

·         Effectively communicate with stakeholders, especially around experimental design, troubleshooting, and responding to changes in priorities and adjusting timelines.

 

Qualifications:

·         Bachelor’s Degree or equivalent education and typically 7 years of experience,

·         Master’s Degree or equivalent education and typically 5 years of experience in molecular biology, genetics, genomics or a related field

·         Demonstrated record of high-proficiency, hands-on molecular biology experience with PCR-based assays and technical knowledge of high-throughput genomic profiling technologies with emphasis on Illumina sequencing platforms and complex library preparation workflows for bulkRNA-seq, 10X Genomics scRNA-seq, and 10X Genomics Visium spatial transcriptomics

·         Knowledge of QC metrics, interpretation, and demonstrated use of approaches for assessing the quality of samples and libraries for bulkRNA, scRNA, and spatial profiling, such as capillary electrophoresis, qPCR, fluorometry, spectrophotometry, cell counting and viability

·         Proficiency with basic bioinformatics tools and sequencing data analysis concepts, such as Illumina SAV, bcl2fastq/BCLConvert, fastQC, and working in a UNIX HPC environment.

·         Experience with 10X Genomics Cellranger and/or Spaceranger software is highly preferred.

·         Experience being part of a centralized high-throughput genomic lab is highly desirable.

·         Industry experience is preferred but willing to consider individuals with equivalent years of experience in academic or non-industry research core lab settings.

·         High attention to detail, self-motivated, critical thinking, strong written and verbal communication skills

·         Ability to work in fast-paced, dynamic environment both collaboratively and independently

·         Proven direct end-to-end hands-on experience with 10X Genomics technologies such as single-cell RNAseq or Visium is highly preferred.

 

Company DescriptionCollabera is a Global Digital Solutions Company providing Software Engineering Solutions for the world's most tech-forward organizations in the areas of Engineering, Cloud and Data/AI. With its roots serving the engineering needs of the world's most recognized businesses in Technology, Financial Services, Telecom and Healthcare, Collabera today operates across 60 locations in 11 countries, serves 30% of the Fortune 500, and has exceeded the industry growth rate by 3-4x for several years.Company DescriptionCollabera is a Global Digital Solutions Company providing Software Engineering Solutions for the world's most tech-forward organizations in the areas of Engineering, Cloud and Data/AI. With its roots serving the engineering needs of the world's most recognized businesses in Technology, Financial Services, Telecom and Healthcare, Collabera today operates across 60 locations in 11 countries, serves 30% of the Fortune 500, and has exceeded the industry growth rate by 3-4x for several years.

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