Associate Scientist II orScientist I, CMC Drug Substance Cell Line Development Group

4 weeks ago


South San Francisco California, United States AbbVie Full time
Job Description

The Development Sciences Biologics CMC Drug Substance Cell Line Development Group in South San Francisco, CA is seeking a Scientist to join a talented and collaborative team developing CHO cell lines for GMP applications.

In this laboratory position, the candidate will perform hands-on molecular characterization studies in a well-supported and dynamic environment to help advance novel therapies to human clinical studies.  The work will encompass molecular, cellular, and bioprocess development studies relating to the generation and characterization of optimized CHO cell lines for production of our pipeline candidates.

 

 

Key Responsibilities:

Design and generate DNA vector constructs for protein expression (including monoclonal antibodies and antibody-like molecules)Optimize the vector workflow to increase speed and throughput

Perform genetic characterization of CHO cell banks to confirm identity and evaluate quality of transgene DNA and RNA.

Review DNA sequencing data (including NGS), Southern/Northern blots, quantitative PCR results

Perform routine molecular screens of CHO cell lines using ddPCR and MinION, P2 sequencers

Author technical reports that meet expectations of regulatory agencies for all stages from IND to BLA

Present updates at team meetings and collaborate closely with others in the cell line development group.

Actively seek opportunities to optimize or enhance the molecular characterization workflow.

Display effective organization, verbal and written communication skills, and adherence to good documentation practices.

 



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