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Senior Research Scientist
2 months ago
Overview:
POSITION SUMMARY:
Under the guidance of the Principal Investigator (PI) or designated representative, the Senior Research Scientist is responsible for the design and execution of a range of intricate research experiments with a significant level of autonomy.
This role will play a crucial part in the oversight of the research program and will engage in various facets of the research initiatives and laboratory upkeep activities.
Responsibilities:
ESSENTIAL FUNCTIONS:
Independently conceptualize and conduct multiple sophisticated laboratory experiments
Identify and employ all relevant software associated with experiments and equipment utilized
Analyze data, draw conclusions, and determine subsequent actions with a high level of independence
Maintain precise records and document results in accordance with established laboratory, sponsor, and institutional protocols
Review literature, publications, and grant proposals pertinent to assigned projects
Document results in compliance with established laboratory, sponsor, and institutional protocols
Conduct comprehensive data analysis and/or mathematical computations
Maintain a meticulous laboratory notebook in accordance with laboratory practices
Compile and interpret data, presenting it in a format suitable for publication as per PI specifications
Present findings at laboratory/group meetings and other relevant forums
Ensure laboratory supply levels are maintained and order supplies as necessary
Oversee laboratory equipment service contracts and coordinate maintenance of laboratory equipment.
Prepare and label buffers, media, solutions, etc.
Monitor and enforce all safety regulations and infection control standards
Identify and draft standard operating procedures in compliance with Environmental Health and Safety (EHS) regulations specific to the laboratory
Train personnel on IACUC, EHS, and radiation safety protocols
Adhere to all applicable state, federal, institutional, and sponsor policies and agreements
Manage daily laboratory operations, including personnel, procedures, and projects
Identify challenges and propose solutions, implementing appropriate resolutions
Develop and implement new protocols and/or modify existing protocols as necessary, with the approval of the PI
Prioritize and execute multiple tasks or procedures simultaneously with attention to detail
Perform duties and experiments independently
Mentor and train laboratory personnel on proper laboratory practices and techniques
Oversee laboratory expenditures in accordance with budget guidelines provided by the PI or designee
Assist with budgetary and operational aspects of grant and contract proposals
Negotiate with vendors to optimize available resources
Contribute independently to the writing of sections for manuscripts and grant proposals
Direct the activities of research support staff
Perform additional duties as required
Qualifications:
REQUIRED QUALIFICATIONS:
Bachelor's degree in Biological or Natural Science with 5+ years of relevant research experience, or a related Master's degree with 3+ years of relevant research experience.
Strong capability to analyze complex visual data in a quantitative and objective manner
Ability to collaborate effectively within a team environment
PREFERRED QUALIFICATIONS:
Experience with T cell responses to virus-specific immunology and heterologous immunity
Proficiency in T cell sorting, TCR sequencing, and T cell phenotyping
Experience with BD-LSRII and BD-FACSymphony A5-SE cell analyzers
Working knowledge of BCL-2 laboratory
Familiarity with real-time PCR techniques
Experience and expertise fields:
Virology, immunology, microbiology, molecular biology
Experience with PBMC isolation from human blood, CD4/CD8 T cell/B cell sorting, HLA-typing, FACS staining, ELISPOT assays, proliferation assays, and ICS production assays of T cells, NK, and Treg cells.
Proficient in antibody subtyping/profiling; setting up short-term T cell cultures; TCR VA/VB / TCR V gamma delta repertoire analysis
Experienced in freezing-thawing T-B cells, plasma, and serum; quantification of cytotoxic T cells using Chromium 51 release assays; utilizing GC 1000 irradiators
Familiarity with T2 and MLA-144 cell lines; preparation of MHC-Class I tetramers with various streptavidin conjugates
Expertise in using Prism Graph Pad 10 and FlowJo 10 software
ADDITIONAL INFORMATION:
SUPERVISION RECEIVED:
Under the direction of the Principal Investigator or designated representative
SUPERVISION EXERCISED:
Supervise aides, technicians, and associates
ENVIRONMENTAL WORKING CONDITIONS:
Wet chemistry laboratory, with potential exposure to biohazards, radionuclides, toxins, animals, and human specimens.
