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Cell Culture Scientist II
2 months ago
We are seeking a highly skilled Cell Culture Scientist II to join our team at Repligen. As a key member of our bioprocessing team, you will be responsible for developing and optimizing cell culture processes for monoclonal antibody, recombinant protein, and viral vector production.
Key Responsibilities- Cell Culture Process Development: Design, develop, and optimize cell culture processes for batch, fed-batch, and perfusion modes using mammalian cell lines in shake flasks, stirred tank, and single-use bioreactors.
- Analytical Assay Activities: Perform cell culture and associated analytical assay activities to support process development and optimization.
- Troubleshooting and Problem-Solving: Troubleshoot cell culture processes by monitoring cell growth, viability, productivity, and other cell culture metabolites.
- Scientific Reporting and Communication: Issue internal scientific reports and publish articles in peer-reviewed and trade journals. Present talks and posters at scientific conferences.
- Intellectual Property Development: Actively participate in expanding Repligen's IP in the field of filtration-based perfusion cell culture applications by submitting invention disclosures.
- Collaboration and Teamwork: Work with other departments, including product management, field applications, and sales, to accomplish individual and company objectives.
- Education: B.S. Degree in chemical engineering, biological sciences, or applicable field with at least 5 years of industrial experience, or M.S. with at least 3 years of industrial experience in a process or application development setting.
- Experience: Extensive understanding of biological process sciences, upstream process development, including aseptic techniques, and cell culture processes.
- Skills: Experienced hands-on work setting up lab (2-10L) and pilot scale bioreactors, cultivating cells, and optimizing feeding and perfusion strategies.
- Expertise: Experience in monoclonal antibody and recombinant protein upstream process development for therapeutic or vaccine applications.
- Analytical and Problem-Solving Skills: Demonstrated experience in design of experiments (DoE), strong analytical and problem-solving skills.
- Communication and Interpersonal Skills: Ability to work independently and within multidisciplinary project teams. Excellent oral and written communication abilities and strong interpersonal skills to communicate with internal and external team members.
- ATF Technology Experience: Experience with ATF technology.
- Viral Vector Experience: Experience in viral vector (AAV, Adenovirus, Lentivirus) upstream process development.
- Perfusion Cell Culture Experience: Experience with perfusion cell culture using ATF technology is recommended.
- Membrane-Based Filtration Understanding: Understanding of membrane-based filtration, including hollow fibers, determining critical flux, shear, and TMP measurements.
