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Research Scientist III

2 months ago


Seattle, Washington, United States HDT Bio Full time
About HDT Bio

HDT Bio is a pioneering biotechnology company that is revolutionizing the field of cancer and infectious disease treatment. Our cutting-edge technologies are designed to harness the power of the host immune system to prevent and cure diseases.

Job Summary

We are seeking a highly skilled Research Associate III - Molecular Biology to join our team. The successful candidate will be responsible for working on the development and characterization of our RNA technology. This is an exciting opportunity to work closely with a talented team of molecular biologists, virologists, immunologists, and material scientists to optimize formulation and delivery of RNA for various applications.

Key Responsibilities
  • Nucleic acid manipulation methods including:
  • Plasmid/construct design
  • PCR and molecular cloning; transformation of E. coli
  • DNA purification and preparation for RNA transcriptions
  • RNA transcription and capping; downstream purification via HPLC
  • RNA analysis methods including:
  • SHAPE-directed RNA structure determination
  • 5'/3-RACE
  • Sequencing
  • Gel and capillary electrophoresis
  • Dot blots
  • Northern blots
  • Nucleic acid transfection and assays for gene transcription and translation including:
  • Western blots and immunofluorescence
  • qRT-PCR, in situ hybridization, nanostring
  • Maintenance of multiple cell-lines, including primary cells
  • Monitor laboratory supply inventories and work with purchasing agents to ensure available stock for group or area.
  • Presentation of research findings at group meetings.
  • Communication with research management on a regular basis.
  • Maintain the highest standards of laboratory practice in line with HDT policy, including maintenance of laboratory notebook.
  • Contribute freely to the team research environment in a manner conducive to the success of the research project as a whole
Requirements
  • Excellent oral and written communication skills
  • Computer literacy, proficiency in MS Office programs
  • Demonstrated expertise in cloning and analysis of DNA
  • Preferred experience includes:
  • RNA handling and analysis
  • HPLC purification of nucleic acids
  • High-throughput screening of nucleic acid libraries for function in vitro
  • Molecular virology
Education/Experience
  • BS/MS in a biological science with 4+/2+ years' experience, respectively
  • 2-4 years full-time laboratory experience with basic molecular cloning, PCR, and cell culture
  • Background in molecular biology

This is a challenging and rewarding opportunity to join a dynamic team of scientists and engineers working on cutting-edge technologies. If you are a motivated and talented individual with a passion for molecular biology, we encourage you to apply.