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Laboratory Technician, Limited Term
3 months ago
The PI is looking for a research technician with experience in molecular biology, cell culture, and laboratory mouse work, to participate in an NIH-funded project. The successful candidate will perform cell culture studies, conduct in vivo experiments in mouse models, analyze mouse tissue at a molecular level, and present data. The candidate will need to be methodical when carrying out duties and generate reports to submit to the laboratory PI.
The job is on site at Chapman University School of Pharmacy, in Irvine, California.
Applicants must be authorized to work for ANY employer in the U.S. We are unable to sponsor or take over sponsorship of an employment Visa at this time. Responsibilities
- Perform PCR, Western blot, ELISA, immunostaining, immunohistochemistry, and other techniques of molecular biology as needed.
- Maintain mammalian cell lines, perform transfections, and collect samples. Perform bacterial transformation and plasmid purification.
- Perform mouse handling, tattooing, tail sampling, and genotyping.
- Determine mouse body temperature (rectal thermometry).
- Mouse blood collection, mouse behavior analysis, mouse organ dissection, thyroidectomy, and mouse brain sectioning.
- Able to perform laboratory experiments in line with standard testing procedures.
- Histology sample preparation, confocal microscopy, data analysis, and presentation.
- Following overall research objectives, independently designs, plans, conducts, and troubleshoots experiments.
- Evaluate protocols and data obtained from groups of experiments and recommend changes to processes, procedures, and techniques to improve the quality and validity of results based on overall project objectives.
- Helps with lab supplies ordering and with lab maintenance.
- Set up, operate, clean, sterilize, maintain, and calibrate laboratory equipment.
- Ensure that safety guidelines are always adhered to in the laboratory.
- Organize and store all chemical substances, fluids, and compressed gases according to safety instructions.
- Procure, receive, label, and/or maintain chemicals or laboratory supplies.
- Be able to work individually and with a scientific team.
- Perform other duties as assigned.
- A bachelor's degree in biology, biochemistry, or a related field plus working experience with cell lines, and/or primary culture.
- Proficient in research methods, testing and data collection, analysis, evaluation, report writing, independent discretion, and judgment.
- Comfortable handling potentially dangerous substances (flammable liquids, biohazards, etc.)
- Ability to work autonomously and in a timely manner to meet deadlines.
- Excellent verbal and written communication skills.
- Experience in Western blot, transfection, transformation.
- Experience in histology, confocal microscopy, mouse behavior analysis, mouse body temperature (rectal thermometry).
The University is committed to achieving a diverse faculty and staff and encourages members of underrepresented groups to apply. More information on diversity and inclusion at Chapman University can be found at www.chapman.edu/diversity.
Applicants for Staff and Administrator positions must be currently authorized to work in the United States on a full-time basis. Chapman University does not sponsor applicants for Staff and Administrator positions for work visas.
The offer of employment is contingent upon satisfactory completion and outcomes of a criminal background screening and returning to the Office of Human Resources a signed original acceptance of the Chapman University Agreement to Arbitrate. Minimum Number of References Maximum Number of References