Scientist

4 weeks ago


Cambridge, United States The Fountain Group LLC Full time

The Fountain Group is currently seeking a Scientist for a prominent client of ours. This position is located in ­­Cambridge, MA. Details for the position are as follows:

Job Description:

  • BioMedicine Design (BMD) High-Throughput Protein Production (HTP) Group is seeking a highly motivated scientist to support the molecular biology & protein production workflows: DNA construct design/assembly, PCR, sample preparation for sequencing, DNA production & quantitation, mammalian cell culture & transfections, protein purification & quantitation, and automated liquid handling.
  • The candidate will play a major role in a core group that consistently delivers hundreds of protein samples per week to research teams across the department.
  • The candidate will be responsible for generating DNA constructs and performing high-throughput processes for macromolecule production (DNA, RNA, and protein).
  • The candidate will also be responsible for updating the laboratory database and relaying data in organized presentations to project teams.
  • ROLE RESPONSIBILITIES
    • DNA plasmid design & cloning
    • Automated high throughput DNA purification
    • High throughput molecular biology operations, including PCR, ligation reactions (Gibson & ITA), & DNA purification using liquid handling automation
    • Sample preparation for sequencing
    • DNA & RNA quantitation & QC
    • Supporting transient mammalian transfections with DNA
    • Organization and storage of DNA fragments/plasmids, including tracking using Genedata Biologics Software, as well as record keeping using electronic laboratory notebooks
    • Close interaction with project teams
    • Regular review of literature to maintain knowledge of scientific trends and industry processes, advance molecular biology and protein production methods

Qualifications:

  • AS or BS
  • Molecular Biology skillset
  • PCR experience
  • Preferred:
  • PREFERRED: Ligation reactions (Gibson & ITA) + DNA purification using liquid handling automation

Compensation: $20-34 an hour depending on experience

 


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