Scientist
3 weeks ago
Responsibilities
* Contribute to the generation of stable cell lines for vaccine and therapeutic protein candidates including monoclonal antibodies, recombinant proteins, and virus-like particles.
* Independently execute each step of the cell line development process including plasmid production, transfection, single cell cloning, clone expansion in static culture plates and shake flasks, fed-batch analysis, and clone stability studies.
* Use automated platforms for clone selection and screening.
* Prepare plasmid DNA using standard molecular cloning techniques.
* Participate in cell line development platform design, optimization, and cycle-time reduction studies including methods for clone selection, expansion, ranking, and characterization.
* Perform small-scale process development studies using various culture vessels including tissue culture plates, shake flasks, and small-scale bioreactors.
* Generate stable pools and perform transient protein production for supply of reagent-grade material.
* Provide laboratory support for medium preparation and ordering of supplies.
* Perform maintenance of cell counters and bioprocess analyzers, coordination of service visits, and regular ordering of consumables.
* Train other laboratory personnel on use of equipment and corresponding processes.
* Write and review technical reports documenting process development studies and stable cell line generation work.
* Work with other team members to generate experimental plans for process development studies.
* Write and review standard operating procedures for cell line development processes and instrumentation.
* Maintain complete documentation records, including a detailed and up-to-date laboratory notebook, to support regulatory filings.
* Regularly communicate progress to team lead and present work to cell line development team .
Experience Required / Requirements
* B.S. in Biological Sciences with a minimum of 5 years of relevant work experience or M.S. in Biological Sciences with independent research experience in an academic or industrial setting.
* Hands-on experience with aseptic technique and mammalian cell culture along with supporting technologies for cell line engineering including cell counting, high-throughput transfection, single-cell cloning, and flow cytometry.
* Experience in preparing technical reports, standard operating procedures, and data presentations.
* Ability to work independently to execute defined experiments and troubleshoot issues as needed to meet project timelines.
* Ability to effectively communicate plans, results, challenges, and ideas to other team members in oral and written forms.
* Familiarity with computer software for word processing and data analysis.
* Experience in monoclonal antibody and/or vaccine production.
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