Research Assistant I NEUROLOGY Khurana Lab

2 weeks ago


Cambridge, United States Brigham and Women's Hospital Full time

The candidate will work independently under the supervision of Vikram Khurana, MD, PhD, and a senior researcher in the Khurana lab (instructor/assistant professor). Depending on the specific project needs and candidate skill set, the role will range from molecular biology focused bench work to development and optimization of assays for viability/toxicity in iPSC (induced pluripotent stem cells) derived neurons. These assays will include techniques such as flow cytometry, longitudinal imaging of neurons, plate-based live cell and calorimetric assays and western blotting. The candidate is expected to be diligent, highly organized, and collegial.

PRINCIPAL DUTIES AND RESPONSIBILITIES:

1. Maintenance, passaging, cataloguing, and culturing of iPSC cells.

2. Differentiation iPSCs to neurons and glia and aging of these cultures.

3. Maintenance of Attune flow cytometry and ordering the related reagents

4. Measuring and scheduling of longitudinal imaging with Incucyte and Nikon Biostation microscope

5. Maintenance of HEK293 cells, generation of stable cell lines.

6. Helping with molecular cloning; Gibson assembly, gateway cloning, mini/maxi preps

7. The candidate will analyze data from western blot/ microscopy/ flow cytometry

8. The candidate will have excellent lab note-keeping habits, mostly digital (LabArchieves) and will generate Standard Operating Procedures (SOPs) for the lab

9. The candidate will have excellent organizational skills, labeling of experiments, file management,

10. The candidate will report daily to the immediate supervisor and weekly to the Principal investigator.

11. The candidate will interact with junior and senior member of the lab to troubleshoot experiments.

12. The candidate will share lab duties with other technicians.

13. Other assignments might be given depending on the project needs.

QUALIFICATIONS:

1. Bachelor of Science degree

2. Good cell culture experience with human cells (HEK293 or any other immortalized line)

3. iPSC stem cell culture experience is a plus but not required.

4. Excellent cell culture practice/sterile techniques are necessary.

5. Medium to high level of computer literacy and a basic understanding of statistical concepts are needed.

6. Basic understanding of molecular techniques, microscopy, flow cytometry is desired.

7. Understanding of basic molecular concepts: molecular cloning, western blot, RNA isolation, qPCR, western blot is desired.

SKILLS/ ABILITIES/ COMPETENCIES REQUIRED:

* 1. Excellent oral and written communication skills.
* 2. High interpersonal skills are required for working in a highly diverse lab.
* 3. Excellent organizational skills and ability to prioritize a variety of tasks.
* 4. Excellent data management skills and attention to details.
* 5. Good presentation skills are a plus.



  • Cambridge, United States Harvard University Full time

    Basic Qualifications College coursework in biology, and 1 year of lab experience. Education may count towards experience. Additional Information To be considered for this position, a resume and cover letter are required. This is a 6-month term position with the possibility of extension based on funding. Harvard University will not provide visa...


  • Cambridge, United States Partners Healthcare System Full time

    Working under general supervision from lab members, executes protocols involving a variety of routine and non-routine experiments at a professional level. Troubleshoots and implements modifications on existing techniques, as appropriate. May suggest alternate technical routes and suggest options based upon research in the literature. Calculates,...


  • Cambridge, United States Brigham and Women's Hospital Full time

    This position will be supporting both the Anderson Lab at Brigham and Women's Hospital as well as the Transcriptomics Platform of the Gene Lay Institute of Immunology and Inflammation. In the Anderson lab, this position will work under minimal supervision to execute research experiments involving a variety of wet-lab techniques including genomics e.g....


  • Cambridge, United States Harvard University Full time

    Unleash your potential in the exciting world of plant diversity research! The Davis Lab, spearheaded by Charles Davis, is a vibrant hub of innovation, including a diverse team of graduate students, postdoctoral fellows, and researchers. Our research Research, Assistant, Diversity, Technology, Education


  • Cambridge, United States Partners Healthcare System Full time

    We are seeking a talented undergraduate coop student to work as an organic and medicinal chemist in our lab in Cambridge, MA. Working under the general supervision of the Lab Director and from experienced lab members, the successful candidate will conduct a variety of organic chemistry synthesis experiments to prepare compounds for biological testing. This...


  • Cambridge, United States Massachusetts Institute of Technology Full time

    REQUIRED: bachelor's degree in biology, biochemistry, or a field directly related to the work; at least two years of experience in a biomedical or biochemical/biological research laboratory; experience in at least one of the following--mammalian cell culture, mouse colony maintenance, iPSC culture, and/or small molecule screening; proficiency with basic lab...

  • Research Scientist I

    2 weeks ago


    Cambridge, United States Broad Institute Full time

    Description & Requirements The Sabeti Lab (, Harvard University, Broad Institute) and The Shalek Lab (, MIT, Broad Institute, and Ragon Institute) have a joint opening for a highly motivated Research Scientist I interested in studying host-pathogen interactions at a single-cell resolution. Research directions will center on interrogating the dynamic...


  • Cambridge, United States Brigham and Women's Hospital Full time

    GENERAL SUMMARY: The Research Engineer I will join the Traverso lab at Brigham and Women's Hospital to support translational development of novel therapeutic technologies. The lab consists of a multidisciplinary team of engineers, scientists and physicians focused on preclinical and translational development of drug delivery technologies that push the...


  • Cambridge, United States Massachusetts Institute of Technology Full time

    SENIOR RESEARCH SUPPORT ASSOCIATE, FLAVELL LAB, Picower Institute for Learning and Memory, to work on research aimed at understanding how neural circuits generate complex behaviors (navigation, state-dependent behaviors, and more) and participating i Research, Associate, Support, Neuroscience, Senior, Technology, Education

  • Research Assistant

    2 weeks ago


    Cambridge, United States Harvard University Full time

    Basic Qualifications College background or equivalent experience. Additional Information This is a one year term appointment with potential for renewal subject to funding and departmental need. We regret that Harvard Law School is unable to provide visa sponsorship for staff positions. All offers to be made by HLS Human Resources. Position...


  • Cambridge, United States Mass General Brigham Full time

    Clinical Research Coordinator II BWH Neurology-(3286736) Description Working under general supervision from manager or PI, providing support to clinical research studies. Independently coordinates all activities of clinical trials and clinical research including but not limited to supervising the daily activities of research assistants, implementing,...


  • Cambridge, United States Massachusetts Institute of Technology Full time

    REQUIRED: bachelor's degree in a related field; two years' experience in laboratory research; experience with research grant writing and reporting processes; ability to understand the laboratory's work and express it verbally and in writing; knowledge of grant funding resources and experience coordinating applications; excellent organizational,...


  • Cambridge, United States Massachusetts Institute of Technology Full time

    REQUIRED: high school diploma or its equivalent; exposure to a research lab; excellent organizational, problem-solving, analytical, time management, and verbal and written communication skills; demonstrated attention to detail; self-direction/motivation; ability to work with colleagues to achieve project goals; practiced documentation skills; awareness of...


  • Cambridge, United States Massachusetts Institute of Technology Full time

    REQUIRED: high school diploma or its equivalent; willingness to work with animals; experience working in a team-oriented environment with people of diverse backgrounds; comfort summarizing and reporting results to researchers; self-motivation; excellent time-management, organizational, communication, and interpersonal skills; commitment to exceeding quality...


  • Cambridge, United States MIT Full time

    **TECHNICAL ASSOCIATE I**, _Koch Institute for Integrative Cancer Research, Lees and Soto-Feliciano Labs_, to work investigating epigenetic regulators and gene regulation in cancer cells. Position will involve some management of research lab operations but the majority of time will be spent conducting research, participating in ongoing lab projects....


  • Cambridge, United States Massachusetts Institute of Technology Full time

    REQUIRED: B.S./B.A. in computer science, software engineering, computer engineering, or related field; at least two years' specialized lab experience (which may include experience acquired as an undergraduate); experience with embedded systems programming; commitment to producing accurate and high-quality protocols and data; ability to independently manage...


  • Cambridge, United States Partners Healthcare System Full time

    GENERAL SUMMARY: The Research Engineer will join the Traverso lab at Brigham and Women's Hospital to develop new pharmacological interventions for chronic diseases (e.g., diabetes, obesity, etc.). The lab consists of a multidisciplinary team of engineers, scientists and physicians focused on preclinical development of translatable technologies that push the...


  • Cambridge, United States Massachusetts Institute of Technology Full time

    RESEARCH TECHNICIAN, Biology-Case Lab, to join a growing research team studying cell signaling. The lab uses quantitative experimental approaches from biochemistry, molecular biophysics, and cell biology to study transmembrane signaling pathways and Technician, Research, Chemistry, Technology, Education


  • Cambridge, United States Massachusetts Institute of Technology Full time

    RESEARCH SUPPORT ASSOCIATE, Koch Institute (KI) for Integrative Cancer Research-Chen Lab, to support a fast-paced and exciting research program in immunology, cancer biology, and metabolic diseases. Will assist with maintaining efficient laboratory o Research, Support, Associate, Equipment Maintenance, Technology, Education


  • Cambridge, United States Massachusetts Institute of Technology Full time

    RESEARCH SPECIALIST, Koch Institute (KI) for Integrative Cancer Research-Koehler Lab, to impact medicine by expanding the repertoire of druggable targets and developing new technological solutions to solve bottlenecks in the drug discovery process. T Research, Specialist, Chemistry, Research Fellow, Technology, Education, Application