Research Assistant 1

3 weeks ago


Cleveland, United States Case Western Reserve University Full time

Job Description

POSITION OBJECTIVE

Working under moderate supervision, the Research Assistant 1 will perform research projects to understand how small molecule metabolites produced by the microbiota, under the influence of host diet, serve as precursor substrates and receptor ligands to regulate metabolism and gene expression in epithelial cells. The lab conducts research in the field of nutritional biochemistry, focusing on how the gut microbiota interacts with the intestinal epithelium to promote tissue homeostasis and how these interactions become altered in pathological states, like inflammatory bowel disease (IBD). This position will work with animals.

ESSENTIAL FUNCTIONS

* Perform laboratory research and carry out protocols as directed by the principal investigator. Studies will involve, but are not limited to, mammalian cell and organoid culture, bacterial culture, PCR/qPCR, transfection and lentivirus-mediated transduction of nucleic acids, Western blotting, isolation of biological molecules (DNA, RNA, protein, metabolites) from cells and tissues, tissue histology, stable isotope tracing by LC-MS/MS, and luciferase assays. Conduct analysis of samples. Experiments may also involve immunofluorescence, NGS for transcriptomics, genomics, and 16S-based microbial community profiling, flow cytometry, and bacterial culture. (60%)
* Perform mouse experiments, including breeding, genotyping, oral gavaging, and tissue collection. (24%)
* Record results for a particular experiment or closely related series of experiments. Maintain clear, accurate, and detailed records of experimental procedures, results, and conclusions. (10%)
* As needed, assist with general organization and maintenance of the laboratory; order supplies, manage inventories, and maintain test equipment utilized in experiments. (6%)

NONESSENTIAL FUNCTIONS

Perform other duties as assigned. (


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