eTeam | Postdoctoral Researcher | cambridge, ma

2 days ago

cambridge, United States eTeam Full time

Position Title: Scientist Level I

Duration: 1 Year

Location: Cambridge, MA

100% ON SITE-Paid parking

Pay Rate Range: $55.00/hr- $58.00/hr on W2


Must-Have:

• PhD in Chemistry, Biology, Biological Engineering, or a related field

• 1 year of professional work experience (in biotech or pharma)

• Protein and antibody engineering workflows, including primary sequence design, expression, purification, characterization

• Bioconjugate chemistry, also referred to as: antibody conjugate experience ADJ

• Strong analytical bio chem skills

• SDS page, chromatography, HPLC


Preferred Skills:

• Previous experience with: (liquid handler, automated buffers) – biochemical assays (nice to have)


Computational skills:

• Computational and AI/ML approaches for rational protein engineering, including AI 3D structure prediction, homology modeling, antibody/antigen docking, other relevant experience

• L&P Experience


Summary:

Client is seeking a highly motivated Scientist I or Scientist II to join the Non-Viral Gene Therapy (NVGT) Team. The successful candidate will have hands-on experience in antibody and protein engineering, with a focus on bioconjugation chemistry. The candidate will work closely with the LNP formulation team to achieve extrahepatic delivery of LNPs.


Responsibilities:

• Execute protein conjugation reactions for both screening efforts and developability to enable nanoparticle targeting to specific cells and/or tissues.

• Perform downstream analytical assays such as SDS-PAGE, SEC-HPLC, RP-HPLC, BLI, DSF, CE-SDS and/or flow-cytometry.

• Perform incorporation of targeting antibody into LNPs and execute associated analytical assays, including DLS, zeta potential, nucleic acid content, endotoxin

• Design protein and antibody molecules at the primary sequence level

• Support management of CRO for molecule production

• Closely work with therapeutic area biologists and project teams to design functional experiments and interpret data

• Execute and carefully document experiments using electronic laboratory notebook system and clearly communicate results to team members.

• Plan, execute, and record experiments as guided in a time sensitive manner.

• Assume responsibility for critical reagent sourcing and maintain adequate supply and inventory.

• Author standard operating procedures, study plans and reports and present findings.

• Foster creativity, productivity, execution with a sense of urgency, scientific integrity and accountability. Maintains a collaborative culture.


Requirements:

• PhD in Chemistry, Biology, Biological Engineering, or a related field with a minimum of 1-3 years of relevant industry experience.

• Strong experience with protein and antibody engineering workflows, including primary sequence design, expression, purification, characterization

• Direct experience with site-specific bioconjugate chemistry

• Strong analytical biochemistry experience for protein and antibody engineering

• Proficient in performing structure-function analysis and bioinformatic analysis of antibodies, antigens, and other proteins

• Experience with automation and high throughput platform(s)

• Experience with Biolayer Interferometry or equivalent technique

• Preferred use of computational and AI/ML approaches for rational protein engineering, including AI 3D structure prediction, homology modeling, antibody/antigen docking, other relevant experience

• Preferred familiarity with formulation and analysis of lipid nanoparticles for research use

• Outstanding written and oral communication skills along with the ability to work collaboratively in a team setting.

• Demonstrated knowledge and skills in solving scientific problems, ability to troubleshoot, and willingness to learn.

• Demonstrated ability to independently design, execute, interpret, and present experimental results.

• Flexibility to adapt to rapidly changing priorities and deadlines in a dynamic, fast paced industry and work environment.

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