Laboratory Technician

(ID: 2024-5180)

Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).

Axle is seeking a Laboratory Technician to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute of Neurological Disorders and Stroke (NINDS) in Bethesda, MD

Benefits We Offer:

• 100% Medical, Dental & Vision Coverage for Employees
• Paid Time Off and Paid Holidays
• 401K match up to 5%
• Educational Benefits for Career Growth
• Employee Referral Bonus
• Flexible Spending Accounts:
  • Healthcare (FSA)
  • Parking Reimbursement Account (PRK)
  • Dependent Care Assistant Program (DCAP)
  • Transportation Reimbursement Account (TRN)

Overall Position Summary and Objectives

Laboratory Technician for NIH/NINDS/Viral Immunology and Intravital Imaging Section (VIIIS) to perform a variety of laboratory techniques and procedures, especially managing mice colonies.

Deliverables:

• Work products and documents related to performing laboratory techniques and procedures, including handling mice and infectious agents, performing immune cell isolations, immunohistochemistry, tissue sectioning, fluorescence and bright field microscopy. - Daily
• Clean Equipment - Weekly
• Work products and documents related to performing digital flow cytometry, ELISAs, DNA/RNA isolations, PCR, RT-PCR, quantitative PCR and Western blots - Weekly
• Work products and documents related to working with staff on the maintenance of transgenic mouse colony, cell lines, logs and database. - Daily
• Work products and documents related to inventory supplies and equipment; prepare purchase requests. - Weekly

Work Details:

• Perform a variety of laboratory techniques and procedures, including handling infectious agents, performing immune cell isolations, immunohistochemistry, tissue sectioning, fluorescence and brightfield microscopy 1
• Work with staff on the maintenance and screening of transgenic mouse colony 2
• Work with staff on the maintenance of laboratory cell lines 3
• Plans, executes, and arranges laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
• Perform digital flow cytometry, ELISAs, DNARNA isolations, PCR, RT-PCR, quantitative PCR and Western blots
• Work with staff on the maintenance of laboratory logs and databases as well as prepare inventory of laboratory supplies and equipment, make recommendations, and prepare purchase requests
• Operates laboratory machines and instruments; and prepares specimens and equipment for use.
• Prepare materials and equipment for experiments.
• Process and organize multiple samples simultaneously.
• Work with a variety of routine and specialized laboratory instruments and equipment in the laboratory.
• Ensure that all specimens and equipment necessary for experiments are available and in good order.
• Prepare specimens and equipment for use in the laboratory.
• Performs sample management and storage.
• Store new samples, update database, retrieve samples with proper authorization.
• Perform processing, description and analysis of samples.
• Work with staff to implement storage and distribution of biological samples for future scientific research.
• Analyzes, documents and reports on all procedures performed.
• Convey and discuss research results with team lab members.
• Collect and enter research data into laboratory notebooks and databases.
• Collect data and prepare for analysis and interpretation.
• Maintains inventory of laboratory supplies.
• Ensure that supplies and consumables are ready and on hand as necessary for maximum efficiency in the laboratory.
• Cleans and sterilizes instruments, glassware and equipment.
• Ensure that adequate clean inventory is available for immediate usage.
• Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
• Perform fundamental procedures required for research projects.
• Perform a variety of laboratory techniques and procedures.
• Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence/immunohistochemistry.
• Perform experiments on samples according to protocols.
• Perform routine experiments and tests; follow established procedures and protocols.
• Maintains inventory of laboratory supplies and initiates purchase orders.
• Work with staff to maintain stocked supplies and records of working reagents, including fixatives, buffers, solvents and staining.
• Ensure that reagents are made correctly and that expired reagents or defective products are replaced to ensure validity of results.
• Order and maintain laboratory supplies, reagents and equipment.
• Performs routine equipment maintenance.
• Assist in troubleshooting and calibrating various laboratory equipment and instruments.
• Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
• Perform a variety of laboratory techniques and procedures.
• Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence/immunohistochemistry.
• Perform routine experiments and tests; follow established procedures and protocols.
• Analyzes, documents and reports on all procedures performed and produces detailed reports for use by senior staff.
• Collect, analyze and interpret data; prepare summaries and reports based on results and findings.
• Record details of experimental procedures, results and protocols.
• Performs advanced equipment maintenance.
• Assist in troubleshooting and calibrating various laboratory equipment and instruments.
• Trains staff in performing routine laboratory tests and procedures.
• Provide technical help and expertise to lab members.
• Work with staff to monitor and ensure that appropriate laboratory safety courses and certifications for staff are up to date.

1, 2, 3, 4, 5 represents priority rankings, where 1 is highest priority and 5 is lowest priority of those ranked

Minimum Education

Additional Qualifications:

• Bachelor's degree in Biology, Life Sciences or a related discipline
• Our Field of Study is Neuroimmunology

• Neuroscience

• NIH POTS
• Microsoft Outlook, Word, Excel, and Powerpoint

• Perform a variety of laboratory techniques and procedures, including handling infectious agents, performing immune cell isolations, immunohistochemistry, tissue sectioning, fluorescence and brightfield microscopy
• Maintain laboratory cell lines
• Maintain and screen transgenic mouse colony

Disclaimer:The above description is meant to illustrate the general nature of work and level of effort being performed by individuals assigned to this position or job description. This is not restricted as a complete list of all skills, responsibilities, duties, and/or assignments required. Individuals may be required to perform duties outside of their position, job description or responsibilities as needed.

The diversity of Axle's employees is a tremendous asset. We are firmly committed to providing equal opportunity in all aspects of employment and will not tolerate any illegal discrimination or harassment based on age, race, gender, religion, national origin, disability, marital status, covered veteran status, sexual orientation, status with respect to public assistance, and other characteristics protected under state, federal, or local law and to deter those who aid, abet, or induce discrimination or coerce others to discriminate.

Accessibility: If you need an accommodation as part of the employment process please contact: careers@axleinfo.com