Sr. Research Assoc/Assoc Scientist, Discovery Biology

3 weeks ago


San Diego, United States Zentalis Full time
Job DescriptionJob DescriptionSalary: $90-125k

JOB SUMMARY:

Responsible for designing and conducting research studies to support our drug discovery programs, including target validation, structure-activity relationship assessment, biomarker discovery, and mechanism of action studies. In addition, evaluates the effects of novel compounds in cancer models (in vitro & in vivo). Hands on, laboratory focused role.

 

ESSENTIAL DUTIES AND RESPONSIBILITIES:

  • Maintain cancer cell lines and establish stable/drug resistant cell lines.
  • Perform semi high-throughput assays and prepare/maintain necessary assay reagents.
  • Participate in target validation.
  • Develop and conduct various cell-based assays to characterize potency of drug candidates.
  • Develop and validate new assays & technologies.
  • Develop other molecular biology-based assays using techniques such as cloning, DNA/RNA/protein extraction and RT-PCR.
  • Analyze, interpret, and generate data reports in a timely manner.
  • Troubleshoot complex assays.
  • Other projects and assignments as required.

 

EDUCATION/KNOWLEDGE/SKILLS/ABILITIES REQUIRED:

  • BS/MS with 5-8 years of experience (pharmaceutical/biotech industry)
  • Knowledge of cancer biology, signal transduction pathways and transcription regulation.
  • Hands-on experience with cancer cell culture and cell-based assays with a variety of readout technologies in low, medium, and high throughput. Experience in spheroid /organoid culture is desirable.
  • Experience with cell genetic manipulation techniques, such as siRNA, shRNA, and CRISPR is desirable.
  • Highly experienced in protein assay development such as western blot/JESS, flow cytometry, ELISA, AlphaLisa, and immunoprecipitation.
  • Experienced in cellular viability assays, and molecular biology techniques, such as cloning and qPCR.
  • In-depth experience of how to use basic software for data analysis (e.g., Prism, Microsoft Excel) and generating reports (PowerPoint).
  • Ability to work independently, multi-task, and prioritize daily activities.
  • High attention to detail.
  • Demonstrates a high level of accuracy and effective oral and written communication skills.
  • Flexibility within a rapidly changing environment.
  • Excellent ability to work in a goal and team-oriented setting.
  • Well-developed organizational skills.


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