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Research Lab Tech I BWH Rheumatology
4 months ago
Principle Duties and Responsibilities:
1. Process samples for research, including:
a. Processing of tissue samples and isolation of cells
b. Isolation and culture of immune and stromal cells from tissue specimens
c. Cell staining for flow cytometry, cell sorting, and mass cytometry
d. Tissue staining for immunohistochemistry and immunofluorescence microscopy
e. Aliquot and freeze or thaw biospecimens
2. Perform functional analyses of stromal and immune cells:
a. Cell stimulation for detection of surface and secreted factors by RT-PCR, ELISA, flow cytometry, western blot
b. Functional assays
c. Co-cultures of different cell types
3. Work independently under general supervision, perform a variety of routine assays and experiments in the laboratory including (but not limited to):
a. Short-term and long-term cell cultures requiring aseptic cell culture technique
b. Functional in vitro cell culture assays
c. Enzymatic activity assays
d. ELISA
e. Flow cytometry
f. PCR/RT-PCR
g. Western blot analysis
h. Immunohistochemistry and immunofluorescence microscopy
4. Perform animal work including (but not limited to) mouse colony maintenance, animal handling, samples collection and processing, genotyping, and carrying out experimental disease models.
5. Report on progress of work at laboratory seminars.
6. Prepare laboratory reagents, chemicals, instruments, and equipment.
7. Maintain supplies inventory in accordance with laboratory and department procedures.
8. Assist in the orientation and training of new students, technicians, and fellows.
9. Maintain established department policies, procedures, and objectives, including the areas of quality assurance, safety, environmental and infection control.
10. Perform other duties as directed.
Qualifications
Qualifications:
- Bachelor's degree or equivalent required.
- Demonstrated organizational and interpersonal skills as required to work closely with a research team.
- Demonstrated competence with databases/spreadsheets and software packages.
- Willingness to learn other laboratory techniques.
- Must possess good organizational skills.
- Ability to communicate information clearly in both oral and written form, including email.
- Ability to make decisions based on logical assumptions of factual data to solve technical and logistical problems.
BWH is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.