Scientist

3 weeks ago


Cambridge, United States Net2Source Inc. Full time

Please find below the job description:-

Job Title: Bench Scientist

Location: Cambridge, MA

Duration: 18 months

Payrate: $30/hr -$34.28/hr. on w2 without Benefits

Job Description: ROLE SUMMARY:

BioMedicine Design (BMD) is seeking a highly motivated Biochemist/Molecular Biologist to join Biotherapeutic Expression and Purification group (BEP) in Cambridge, MA. Qualified candidate will play an essential role in a high-energy DNA production and protein expression research laboratory. The group supports non-GMP transient protein lead production across the companies diverse biotherapeutic discovery portfolio to enable the research units and their pre-clinical therapeutic in-vitro and in-vivo needs. The BEP DNA production and protein expression group is looking for a laboratory technician to provide DNA production support for the protein expression and purification activities in BEP. The candidate is expected to have a strong background in molecular biology techniques and experience with nucleic acid production and analysis. The successful candidate will grow bacterial cultures and perform large-scale DNA production activities following standardized protocols. The candidate will be a key contributor to the scientific team responsible for protein expression and purification.

Role Responsibilities

• Production of plasmid DNA to support transient recombinant protein expression.

• Conducting routine molecular biology techniques including cell transformations, day cultures, bacterial cultures, Gibson assembly, and digestions.

• Maintaining reagent and DNA inventory.

• Operate and maintain automated liquid handlers for plasmid purification, as well as downstream QC applications like NGS and DNA normalizations.

• Conducting routine DNA characterization and quality control testing using techniques such as gel electrophoresis, fragment analysis, nanodrop, endotoxin testing, and preparation of samples for sequencing analysis (Sanger and NGS).

• Maintaining daily work records in an organized electronic lab notebook. The candidate should also be proficient in using an online database for inventory management.

• Effectively communicating with team members to meet strict schedules and deadlines

Required Skills:

• Production of plasmid DNA

• Conducting routine molecular biology techniques

• Maintaining reagent and DNA inventory.

Preferred Skills:

• Handling Automations

• Familiarity NGS "Nanopore "

Nice to Have

• Familiarity with Golden Gate or Gibson assembly


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