Scientist I, Downstream CMC

3 months ago

Boston, United States Entrada Therapeutics Full time
The Organization

What's happening at Entrada Therapeutics? Entrada is a clinical-stage biopharmaceutical company aiming to transform the lives of patients by establishing a new class of medicines that engage intracellular targets that have long been considered inaccessible.

Our Endosomal Escape Vehicle (EEV™)-therapeutics are designed to enable the efficient intracellular delivery of a wide range of therapeutics into a variety of organs and tissues, resulting in an improved therapeutic index. Through this proprietary, versatile and modular approach, Entrada is advancing a robust development portfolio of RNA-, antibody- and enzyme-based programs for the potential treatment of neuromuscular, ocular, metabolic and immunological diseases, among others.

Our lead oligonucleotide programs are in development for the potential treatment of people living with Duchenne who are exon 44, 45 and 50 skipping amenable. We have also partnered to develop a clinical-stage program, VX-670, for myotonic dystrophy type 1.

We are a tight-knit team of experts and leaders in both therapeutic development and rare diseases and are excited to grow and attract colleagues who are ready to join a high-energy, dedicated team that likes to get things done and dramatically improve the lives of patients and their families.The Perfect Addition to Our Team

You are versatile, self-motivated, and looking forward to rolling up your sleeves to contribute to the overarching goals. You enjoy a fast-paced, highly dynamic environment where you’ll learn and grow in the role. You follow up and follow through, connecting problems with solutions efficiently. You are a good communicator and enjoy collaborating with internal and external stakeholders. The Opportunity

The Scientist I, Downstream CMC will support the company on a critical growth trajectory. Conducting high throughput screens and developing downstream chromatography, including but not limited to Affinity, HIC, IEX, SEC, and MMC. They will perform and lead these activities with minimal supervision. The ideal candidate will interact across different functional groups, including Discovery and Pharmacology. Your ability and desire to thrive in a nimble, fast-paced, results-driven environment will set you up for success. Responsibilities

  • Perform high-throughput screening of different chromatography resins/columns and identify purification conditions amenable to scale-up for recombinant proteins and protein-conjugates.
  • Execute primary separations (homogenization, solubilization, refold), purification, and formulation.
  • Accountable for designing experimental plans for purifying recombinant proteins and protein conjugates.
  • Execute identified experimental plans independently with minimal supervision, analyze results, and communicate them effectively in group meetings.
  • Coordinate with Upstream, Analytical, and Biologics Discovery groups to complete downstream activities.
  • Develop and generate protocols, procedures, and records to support high throughput screening.
  • Ensure well-organized, clear, and complete records of all experimental activities in laboratory notebooks promptly.
  • Maintain downstream lab equipment and inventory of reagents and consumables in collaboration with other team members. The Necessities

    At Entrada, our passion for science, our devotion to patients and our values drives our behavior:

    • Humanity - We genuinely care about patients and about one another.
    • Tenacity - We are relentless and persistent in the pursuit of developing therapies for patients.
    • Creativity - We are creative problem solvers.
    • Collaboration - We are more than the sum of our parts.
    • Curiosity - We have a growth mindset and push conventional thought and theory.

      To thrive on our team, you will need to come with:

    • PhD in Biochemistry, Biochemical Engineering, or related scientific field with 1+ years of experience in purification and characterization of recombinant proteins.
  • Experience purifying recombinant tagless and tagged proteins, including antibodies and enzymes from various hosts, e.g., E. coli, CHO, and Pichia.
Skillful in identifying & resolving purification issues or proactively optimizing purification methods. Demonstrated evidence in scientific publications/abstracts/posters is expected. Ability to proactively prioritize and flexibly drive experimental designs. Hands-on experience working with deep well plates (resin screening) and AKTA/NGC FPLC systems to purify recombinant proteins using affinity, HIC, MMC, IEX, and SEC chromatography. Experience with TFF operation to concentrate and diafilter recombinant proteins. Knowledge of biologics downstream process development strategies (including inclusion body solubilization, refolding, column chromatography, conjugation, and formulation) and familiarity with best protein purification practices. Excellent communication skills (oral and written) and ability to work effectively across departments. Growth mindset with the ability to adapt to highly changing priorities. Experience in a fast-growing, dynamic organization; preferably small biotech is preferred. Experience in handling high throughput instruments such as protein maker is preferred. The Perks

By becoming a team member here at Entrada, you’ll have access to competitive health, dental, and vision coverage, as well as life insurance, and short term and long-term disability insurance. We value work life balance, you'll benefit from discretionary time off, paternity leave, and an excellent 401(k) package. We also offer a generous transportation stipend to commute as you wish. Our location in the vibrant and growing Seaport District is close to all the best that the city of Boston has to offer.

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law. Third Party Staffing Agencies

Entrada does not accept unsolicited resumes from any source other than directly from candidates. For the protection of all parties involved in the recruiting process, resumes will only be accepted from recruiters/agencies if a signed agreement is in place at the inception of the recruiting effort and authorized for a specified position. Unsolicited resumes sent to Entrada from recruiters/agencies do not constitute any type of relationship between the recruiter/agency and Entrada and do not obligate Entrada to pay fees if we hire from those resumes. Privacy Statement

Entrada Therapeutics, Inc. (the “Entrada,” “we,” “us,” or “our”) respects your privacy and we want you to be familiar with how we collect, use, share, or otherwise process, your Personal Information. Please reference our privacy statement here to understand how and when your data is being used.

  • Scientist, CMC

    4 weeks ago

    Boston, Massachusetts, United States PepGen Full time

    Job OverviewPepGen is seeking a highly skilled Scientist, CMC to contribute to the development of our Enhanced Delivery Oligonucleotides (EDOs) pipeline. As a key member of our CMC team, you will work closely with external partners to develop optimized chromatography processes for purifying target entities.ResponsibilitiesDevelop and implement process...

  • Research Associate I, Downstream CMC

    3 months ago

    Boston, United States Entrada Therapeutics Full time

    The Organization What's happening at Entrada Therapeutics? Entrada is a clinical-stage biopharmaceutical company aiming to transform the lives of patients by establishing a new class of medicines that engage intracellular targets that have long been considered inaccessible. Our Endosomal Escape Vehicle (EEV™)-therapeutics are designed to enable the...

  • Director of Downstream Process Development

    1 week ago

    Boston, Massachusetts, United States EPM Scientific Full time

    Job DescriptionAs a key member of our team, the Director of Downstream Process Development will be responsible for leading the development and optimization of downstream processes for the production of novel biologics. This role requires a strong understanding of chromatography, filtration, and process validation, as well as a strategic vision for developing...

  • Director of Downstream Process Development

    7 days ago

    boston, United States EPM Scientific Full time

    Director of Downstream Process DevelopmentJob Summary:The Director of Downstream Process Development will lead the development and optimization of downstream processes for the production of novel biologics. This role is responsible for design, scale-up, and transfer purification processes from laboratory scale to manufacturing, ensuring compliance with...

  • Director of Downstream Process Development

    7 days ago

    Boston, United States EPM Scientific Full time

    Director of Downstream Process DevelopmentJob Summary:The Director of Downstream Process Development will lead the development and optimization of downstream processes for the production of novel biologics. This role is responsible for design, scale-up, and transfer purification processes from laboratory scale to manufacturing, ensuring compliance with...

  • Director of Downstream Process Development

    1 week ago

    Boston, United States EPM Scientific Full time

    Job DescriptionJob DescriptionDirector of Downstream Process DevelopmentJob Summary:The Director of Downstream Process Development will lead the development and optimization of downstream processes for the production of novel biologics. This role is responsible for design, scale-up, and transfer purification processes from laboratory scale to manufacturing,...

  • Director of Downstream Process Development

    2 weeks ago

    Boston, United States EPM Scientific Full time

    Job DescriptionJob DescriptionDirector of Downstream Process Development Job Summary:The Director of Downstream Process Development will lead the development and optimization of downstream processes for the production of novel biologics. This role is responsible for design, scale-up, and transfer purification processes from laboratory scale to manufacturing,...

  • Senior Scientist

    7 days ago

    Boston, Massachusetts, United States Takeda Full time

    About the RoleAt Takeda, we are a forward-thinking, world-class R&D organization that unlocks innovation and delivers transformative therapies to patients. As a Senior Scientist - Analytical Development, you will provide and drive theoretical/conceptual input to the design, development, and execution of research assignments for a specific project or projects...

  • Head, CMC Radiopharmaceuticals

    7 days ago

    boston, United States Z-Alpha Therapeutics Full time

    Z-Alpha Therapeutics is a San Diego based pharmaceutical company dedicated to the discovery and development of radiotherapeutics targeting key biological pathways for solid tumors. The company has a revolutionary technology platform in the next generation of alpha particle therapy with potential to be a more targeted therapy, increasing efficacy and limiting...

  • Head, CMC Radiopharmaceuticals

    7 days ago

    Boston, United States Z-Alpha Therapeutics Full time

    Z-Alpha Therapeutics is a San Diego based pharmaceutical company dedicated to the discovery and development of radiotherapeutics targeting key biological pathways for solid tumors. The company has a revolutionary technology platform in the next generation of alpha particle therapy with potential to be a more targeted therapy, increasing efficacy and limiting...

  • Director Process Development CMC

    3 weeks ago

    Boston, United States Barrington James Full time

    Director Process Development CMCI am currently partnered with an exciting innovative Cell Therapy Biotech developing derived immune cell therapy products addressing significant opportunities with unmet clinical needs. I am collaborating with the CTO who is expanding the CMC team and currently seeking a Senior Director of Process Development.You will be...

  • Director Process Development CMC

    1 week ago

    boston, United States Barrington James Full time

    Director Process Development CMCI am currently partnered with an exciting innovative Cell Therapy Biotech developing derived immune cell therapy products addressing significant opportunities with unmet clinical needs. I am collaborating with the CTO who is expanding the CMC team and currently seeking a Senior Director of Process Development.You will be...

  • Director Process Development CMC

    3 weeks ago

    Boston, United States Barrington James Full time

    Director Process Development CMCI am currently partnered with an exciting innovative Cell Therapy Biotech developing derived immune cell therapy products addressing significant opportunities with unmet clinical needs. I am collaborating with the CTO who is expanding the CMC team and currently seeking a Senior Director of Process Development.You will be...

  • Director Process Development CMC

    3 weeks ago

    Boston, United States Barrington James Full time

    Director Process Development CMCI am currently partnered with an exciting innovative Cell Therapy Biotech developing derived immune cell therapy products addressing significant opportunities with unmet clinical needs. I am collaborating with the CTO who is expanding the CMC team and currently seeking a Senior Director of Process Development.You will be...

  • Scientist I, Analytical Development Specialist

    2 weeks ago

    Boston, Massachusetts, United States Elektrofi Inc Full time

    Job Title: Scientist I, Analytical DevelopmentElektrofi Inc is a pioneering company in the field of biologic therapies, revolutionizing the delivery of life-changing medicines. We are seeking a highly motivated and detail-oriented Scientist I to join our Process Analytics team within our Analytical Development department.Job SummaryThe successful candidate...

  • Senior Director, GRA CMC Pharmaceuticals Development Portfolio Lead

    4 weeks ago

    Boston, United States Takeda Full time

    By clicking the “Apply” button, I understand that my employment application process with Takeda will commence and that the information I provide in my application will be processed in line with Takeda’sPrivacy Notice and Terms of Use. I further attest that all information I submit in my employment application is true to the best of my knowledge.Job...

  • Senior Director, GRA CMC Pharmaceuticals Development Portfolio Lead

    3 weeks ago

    Boston, United States Takeda Pharmaceuticals Full time

    By clicking the “Apply” button, I understand that my employment application process with Takeda will commence and that the information I provide in my application will be processed in line with Takeda’s Privacy Notice and Terms of Use. I further attest that all information I submit in my employment application is true to the best of my knowledge.Job...

  • Senior Formulations Scientist

    1 week ago

    Boston, Massachusetts, United States Elektrofi Inc Full time

    Job Title: Senior Formulations ScientistWe are seeking a highly skilled Senior Formulations Scientist to join our team at Elektrofi Inc. As a key member of our formulation development team, you will play a crucial role in the development of our ultra-high concentration microparticle suspension products.Key Responsibilities:Lead the development of novel,...

  • Medical Laboratory Scientist I, Molecular

    4 weeks ago

    Boston, Massachusetts, United States Mass General Brigham Full time

    Job Title: Medical Laboratory Scientist I, MolecularJob Summary:We are seeking a highly skilled Medical Laboratory Scientist I, Molecular to join our team at Brigham and Women's Hospital. The successful candidate will be responsible for performing qualitative and quantitative tests and procedures using instruments and/or manual methods on blood, plasma, and...

  • Medical Lab Scientist I

    1 month ago

    Boston, United States Actalent Full time

    Job DescriptionJob DescriptionJob DescriptionResponsibilities are interchangeable within job titles of medical laboratory scientist (MLS), medical technologist, or clinical laboratory scientist. Determine the acceptability of specimens for testing according to established criteria. Perform routine and complex technical procedures and functions according to...