Research Associate I, Downstream CMC

2 months ago

Boston, United States Entrada Therapeutics Full time
The Organization

What's happening at Entrada Therapeutics? Entrada is a clinical-stage biopharmaceutical company aiming to transform the lives of patients by establishing a new class of medicines that engage intracellular targets that have long been considered inaccessible.

Our Endosomal Escape Vehicle (EEV™)-therapeutics are designed to enable the efficient intracellular delivery of a wide range of therapeutics into a variety of organs and tissues, resulting in an improved therapeutic index. Through this proprietary, versatile and modular approach, Entrada is advancing a robust development portfolio of RNA-, antibody- and enzyme-based programs for the potential treatment of neuromuscular, ocular, metabolic and immunological diseases, among others.

Our lead oligonucleotide programs are in development for the potential treatment of people living with Duchenne who are exon 44, 45 and 50 skipping amenable. We have also partnered to develop a clinical-stage program, VX-670, for myotonic dystrophy type 1.

We are a tight-knit team of experts and leaders in both therapeutic development and rare diseases and are excited to grow and attract colleagues who are ready to join a high-energy, dedicated team that likes to get things done and dramatically improve the lives of patients and their families. The Perfect Addition to Our Team

You are a versatile, self-motivated professional who enjoys rolling your sleeves to contribute to the overarching goals. You enjoy a fast-paced, highly dynamic environment where you learn and grow in the role. You follow up and follow through, connecting problems with solutions efficiently. You are a good communicator and enjoy collaborating with internal and external stakeholders. The Opportunity

The Research Associate I, Downstream CMC (Contract) position will support the Company’s intracellular biologics discovery programs. They will work closely with downstream and upstream teams to progress discovery and development programs. The successful candidate will execute the purification of recombinant proteins using AKTA Avant, Protein Maker and Bio-Rad NGC systems. By the end of the contract, the candidate will gain hands-on laboratory experience and a solid practical understanding of downstream operations involved in producing recombinant proteins. Responsibilities

  • Prepare buffers for downstream (homogenization, solubilization, and protein purification) operations.
  • Operate AKTA Avant, Bio-Rad NGC, purifier, and TFF systems for protein purification.
  • Execute homogenization, solubilization, and refolding of inclusion bodies.
  • Clean-in-place AKTA and Bio-Rad systems after use.
  • Run SDS-PAGE gels, western blots, and endotoxin assays.
  • Input experimental details and observations in the laboratory notebooks.
  • Present data and research findings in internal team meetings.
  • Strong communication skills (oral and written) and ability to work effectively across functions.
  • Maintain inventory of reagents and consumables (as needed).
  • Prepare media and feed for shake flasks and fermenters (as needed).
  • Set up, inoculate, and operate shake flasks and lab-scale fermenters (as needed).
  • Sample shake flask and fermenter to monitor culture growth, metabolite levels, and protein production (as needed).
  • Run activity, SEC-HPLC and Ion exchange HPLC assays (as needed). The Necessities

    At Entrada, our passion for science, our devotion to patients and our values drives our behavior:

    • Humanity - We genuinely care about patients and about one another.
    • Tenacity - We are relentless and persistent in the pursuit of developing therapies for patients.
    • Creativity - We are creative problem solvers.
    • Collaboration - We are more than the sum of our parts.
    • Curiosity - We have a growth mindset and push conventional thought and theory.

      To thrive on our team, you will need to come with:

    • BS or MS degree in biochemistry, biochemical engineering, biotechnology, or a related field with prior experience in R&D laboratory.
  • Experience with AKTA or Bio-Rad systems for purification of recombinant proteins using multimodal chromatography, Ion exchange, and other types of chromatography.
Experience with TFF operation to concentrate and diafilter recombinant proteins (a plus). Experience in a fast-growing, dynamic organization; preferably small biotech is preferred. Experience in handling high throughput instruments such as protein maker is preferred. Knowledge and use of molecular biology and protein biochemistry techniques. Able to work outside of regular business hours and weekends. Growth mindset with ability to adapt to changing priorities. Strong communication skills (oral and written) and ability to work effectively across functions. Desire to work in an innovative and collaborative environment pursuing high-reward science. The Perks

By becoming a team member here at Entrada, you’ll have access to competitive health, dental, and vision coverage, as well as life insurance, and short term and long-term disability insurance. We value work life balance, you'll benefit from discretionary time off, paternity leave, and an excellent 401(k) package. We also offer a generous transportation stipend to commute as you wish. Our location in the vibrant and growing Seaport District is close to all the best that the city of Boston has to offer.

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law. Third Party Staffing Agencies

Entrada does not accept unsolicited resumes from any source other than directly from candidates. For the protection of all parties involved in the recruiting process, resumes will only be accepted from recruiters/agencies if a signed agreement is in place at the inception of the recruiting effort and authorized for a specified position. Unsolicited resumes sent to Entrada from recruiters/agencies do not constitute any type of relationship between the recruiter/agency and Entrada and do not obligate Entrada to pay fees if we hire from those resumes. Privacy Statement

Entrada Therapeutics, Inc. (the “Entrada,” “we,” “us,” or “our”) respects your privacy and we want you to be familiar with how we collect, use, share, or otherwise process, your Personal Information. Please reference our privacy statement here to understand how and when your data is being used.

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