Research Lab Tech I BWH Rheumatology

3 weeks ago

Boston, United States Brigham & Women's Hospital(BWH) Full time

This position in the laboratory of Michael Brenner, MD, who is world renowned for his contributions to immunology and arthritis biology, offers the opportunity to join a dynamic group studying inflammation and autoimmunity, with a particular focus on rheumatoid arthritis. Some important advances in the field driven by Dr. Brenner’s research group include the discovery of a unique population of T cells that is dramatically expanded in the joints of rheumatoid arthritis patients, published in the journal Nature, as well as the identification of distinct fibroblast populations within joint tissue of rheumatoid arthritis patients, reported in Nature Communications. This research group seeks to continue to advance our understanding of arthritis through “disease deconstruction,” in which we study the function of cells from patients, including T cells, macrophages, and fibroblasts. These functions are evaluated through a combination of analytical methods, including single cell RNA-sequencing, bulk RNA-seq, flow cytometry, along with in vitro functional studies and animal models. The detailed analyses of tissues from patient samples are carried out in close collaboration with the BWH Human Immunology Center and Single Cell Genomics Core. Under general supervision by a supervisor, and in accordance with established hospital policies and procedures, the self-motivated individual in this position will perform activities related to the processing and analysis of cells and tissue samples from humans and mice using standard immunology and molecular biology techniques, including cell isolation and cell culture, flow cytometry, RT-PCR, and ELISA. Responsibilities also include tracking and organizing tissue and cell samples, developing analysis methods, maintaining regulatory protocols, data analysis, and preparation and presentation of data. In addition, this individual will perform a variety of routine assays and experiments in the laboratory and help in general to maintain lab organization and support laboratory staff. This position offers many opportunities to learn by one-on-one interactions, lab meetings, and group meeting formats. There are also opportunities to present data gathered and be part of discussions of data significance and to learn about a variety of technologies and biologic areas of research in the lab.

Principle Duties and Responsibilities:

1. Process samples for research, including:

a. Processing of tissue samples and isolation of cells

b. Isolation and culture of immune and stromal cells from tissue specimens

c. Cell staining for flow cytometry, cell sorting, and mass cytometry

d. Tissue staining for immunohistochemistry and immunofluorescence microscopy

e. Aliquot and freeze or thaw biospecimens

2. Perform functional analyses of stromal and immune cells:

a. Cell stimulation for detection of surface and secreted factors by RT-PCR, ELISA, flow cytometry, western blot

b. Functional assays

c. Co-cultures of different cell types

3. Work independently under general supervision, perform a variety of routine assays and experiments in the laboratory including (but not limited to):

a. Short-term and long-term cell cultures requiring aseptic cell culture technique

b. Functional in vitro cell culture assays

c. Enzymatic activity assays

d. ELISA

e. Flow cytometry

f. PCR/RT-PCR

g. Western blot analysis

h. Immunohistochemistry and immunofluorescence microscopy

4. Perform animal work including (but not limited to) mouse colony maintenance, animal handling, samples collection and processing, genotyping, and carrying out experimental disease models.

5. Report on progress of work at laboratory seminars.

6. Prepare laboratory reagents, chemicals, instruments, and equipment.

7. Maintain supplies inventory in accordance with laboratory and department procedures.

8. Assist in the orientation and training of new students, technicians, and fellows.

9. Maintain established department policies, procedures, and objectives, including the areas of quality assurance, safety, environmental and infection control.

10. Perform other duties as directed.

  • Research Scientist

    2 weeks ago

    Boston, United States Brigham & Women's Hospital(BWH) Full time

    **Research Scientist - BWH Rheumatology** - (3291658) **General Summary** **Primary Responsibilities** The position requires the ability to develop an in-depth understanding of our research questions as well as the data and data structures. Willingness to learn new skills and ability to work in a team setting is essential. The position includes...

  • Research Lab Tech I BWH Rheumatology

    2 weeks ago

    Boston, United States Mass General Brigham Full time

    Research Lab Tech I BWH Rheumatology -(3289492) Description This position in the laboratory of Michael Brenner, MD, who is world renowned for his contributions to immunology and arthritis biology, offers the opportunity to join a dynamic group studying inflammation and autoimmunity, with a particular focus on rheumatoid arthritis. Some important...

  • Research Lab Tech I BWH Rheumatology

    1 day ago

    Boston, United States Mass General Brigham Full time

    Research Lab Tech I BWH Rheumatology -(3289492) Description This position in the laboratory of Michael Brenner, MD, who is world renowned for his contributions to immunology and arthritis biology, offers the opportunity to join a dynamic group studying inflammation and autoimmunity, with a particular focus on rheumatoid arthritis. Some important...

  • Research Lab Tech I BWH Rheumatology

    1 week ago

    Boston, Massachusetts, United States Brigham & Women's Hospital(BWH) Full time

    This position in the laboratory of Michael Brenner, MD, who is world renowned for his contributions to immunology and arthritis biology, offers the opportunity to join a dynamic group studying inflammation and autoimmunity, with a particular focus on rheumatoid arthritis. Some important advances in the field driven by Dr. Brenner's research group include the...

  • Research Lab Tech I BWH Rheumatology

    1 day ago

    Boston, Massachusetts, United States Brigham & Women's Hospital(BWH) Full time

    This position in the laboratory of Michael Brenner, MD, who is world renowned for his contributions to immunology and arthritis biology, offers the opportunity to join a dynamic group studying inflammation and autoimmunity, with a particular focus on rheumatoid arthritis. Some important advances in the field driven by Dr. Brenner's research group include the...

  • Research Lab Tech I

    1 week ago

    Boston, United States Brigham & Women's Hospital(BWH) Full time

     Job Description - Research Lab Tech I (3291161)   The Cichowski laboratory, a lab within BWH, Harvard Medical School, and the Dana-Farber Harvard Cancer Center, is searching for motivated individuals to fill 1-2 Research Assistant positions. Our laboratory utilizes biochemical, cellular and mouse modeling approaches to elucidate mechanisms that drive...

  • Research Lab Tech I

    1 week ago

    Boston, Massachusetts, United States Brigham & Women's Hospital(BWH) Full time

    Job Description - Research Lab Tech I The Cichowski laboratory, a lab within BWH, Harvard Medical School, and the Dana-Farber Harvard Cancer Center, is searching for motivated individuals to fill 1-2 Research Assistant positions. Our laboratory utilizes biochemical, cellular and mouse modeling approaches to elucidate mechanisms that drive cancer. We then...

  • Research Lab Tech I

    1 day ago

    Boston, Massachusetts, United States Brigham & Women's Hospital(BWH) Full time

    Job Description - Research Lab Tech I The Cichowski laboratory, a lab within BWH, Harvard Medical School, and the Dana-Farber Harvard Cancer Center, is searching for motivated individuals to fill 1-2 Research Assistant positions. Our laboratory utilizes biochemical, cellular and mouse modeling approaches to elucidate mechanisms that drive cancer. We then...

  • Research Lab Tech I, Dept. of Orthopaedics

    2 weeks ago

    Boston, United States Brigham & Women's Hospital(BWH) Full time

    **Research Lab Tech I, Dept. of Orthopaedics** - (3290870) The Dept. of Orthopaedics is looking for an independent and highly motivated individual to join our research program focused on mammalian regenerative biology. The Lehoczky Lab studies mouse digit tip regeneration and is presently focused on determining the molecular pathways necessary for this...

  • Research Lab Tech I, Newborn Medicine

    21 hours ago

    Boston, United States Brigham & Women's Hospital(BWH) Full time

    **Research Lab Tech I, Newborn Medicine** - (3285611) We are seeking a highly motivated and detail-oriented individual to join our dynamic research team as a Research Assistant on-site at Brigham & Women's Hospital. Under the supervision of the Principal Investigator and other lab personnel the Research Assistant will be responsible for facilitating all...

  • Research Lab Tech I BWH Rheumatology

    3 weeks ago

    Boston, Massachusetts, United States Brigham & Women's Hospital(BWH) Full time

    This position in the laboratory of Michael Brenner, MD, who is world renowned for his contributions to immunology and arthritis biology, offers the opportunity to join a dynamic group studying inflammation and autoimmunity, with a particular focus on rheumatoid arthritis. Some important advances in the field driven by Dr. Brenner's research group include the...

  • Research Lab Tech I BWH Rheumatology

    1 day ago

    Boston, Massachusetts, United States Brigham & Women's Hospital(BWH) Full time

    This position in the laboratory of Michael Brenner, MD, who is world renowned for his contributions to immunology and arthritis biology, offers the opportunity to join a dynamic group studying inflammation and autoimmunity, with a particular focus on rheumatoid arthritis. Some important advances in the field driven by Dr. Brenner's research group include the...

  • Research Lab Tech I Endocrinology

    1 day ago

    Boston, United States Brigham & Women's Hospital(BWH) Full time

    **Research Lab Tech I Endocrinology** - (3288998) **GENERAL SUMMARY**: **PRINCIPAL DUTIES AND **RESPONSIBILITIES**: - Under the Supervision of Principal Investigator, designs and performs research laboratory experiments in the field of reproductive endocrinology, including: a. Preparation and analysis of DNA and RNA, including genotyping - b. Isolation...

  • Research Assistant I BWH Neurology Fox Lab

    1 week ago

    Boston, United States Brigham & Women's Hospital(BWH) Full time

     Job Description - Research Assistant I BWH Neurology Fox Lab (3288885)   Working under the supervision of a Senior Research Assistant, Manager, or Principal Investigator and following established policies and procedures, provides assistance on clinical research studies. May be responsible for the following activities: recruiting and evaluating patients...

  • Research Assistant I BWH Neurology Fox Lab

    1 week ago

    Boston, Massachusetts, United States Brigham & Women's Hospital(BWH) Full time

    Job Description - Research Assistant I BWH Neurology Fox Lab Working under the supervision of a Senior Research Assistant, Manager, or Principal Investigator and following established policies and procedures, provides assistance on clinical research studies. May be responsible for the following activities: recruiting and evaluating patients for studies;...

  • Research Assistant I BWH Neurology Fox Lab

    1 day ago

    Boston, Massachusetts, United States Brigham & Women's Hospital(BWH) Full time

    Job Description - Research Assistant I BWH Neurology Fox Lab Working under the supervision of a Senior Research Assistant, Manager, or Principal Investigator and following established policies and procedures, provides assistance on clinical research studies. May be responsible for the following activities: recruiting and evaluating patients for studies;...

  • Research Lab Technician Ll Bwh Allergy

    21 hours ago

    Boston, United States Brigham & Women's Hospital(BWH) Full time

    **Research Lab Technician ll BWH Allergy** - (3292840) Working under the general supervision of Principal Investigators, and in accordance with established hospital policies and procedures, conducts routine and non-routine experiments at a professional level. Laboratory work, analysis of data using statistical techniques. May perform literature searches and...

  • Research Assistant I Bwh Cardiovascular

    1 month ago

    Boston, United States Brigham & Women's Hospital(BWH) Full time

    **Research Assistant I BWH Cardiovascular** - (3287497) Working very independently with little guidance from Project Manager and/or Principal Investigator, coordinates all activities of research area including but not limited to supervising the daily activities of research assistants, implementing and maintaining quality assurance procedures, performing...

  • Research Lab Technician Bwh Cardiovascular

    1 month ago

    Boston, United States Brigham & Women's Hospital(BWH) Full time

    **Research Lab Technician BWH Cardiovascular** - (3287150) General Summary/Overview Statement Under supervision of Principal Investigators/Directors in accordance with established hospital policies and procedures and specific instructions, will perform routine and non-routine laboratory procedures, record tests, assist in laboratory management and safety,...

  • Clinical Investigation Technician I Bwh Sleep

    1 month ago

    Boston, United States Brigham & Women's Hospital(BWH) Full time

    **Clinical Investigation Technician I BWH Sleep Medicine** - (3288129) GENERAL SUMMARY/ OVERVIEW STATEMENT: The Sleep Disorders Research Program is committed to find cutting edge technologies to predict sleep apnea diagnosis and outcome and is paving the way to find pharmacotherapy for sleep apnea. The Sleep Disorder Research Program has considerably...